FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_48.3520000005f47e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_48.3520000005f47e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30370
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA3481.145867632532104No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA530.17451432334540665No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT400.1317089232795522No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA370.12183075403358577No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA370.12183075403358577No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA360.11853803095159697No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.11195258478761937No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA320.10536713862364176No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA320.10536713862364176No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA310.10207441554165296No Hit
ACTATATTGTGTGTTGTTTGTATCCGCTCGCTA310.10207441554165296No Hit
TATCAACGCAGAGTGAATGGGTTCCACAAAAAA310.10207441554165296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC401.7776594E-523.62526
CGTTGAT401.7776594E-523.62523
GATACCA454.415735E-521.027
TTGATAC509.9312165E-518.90000225
GCGTTGA1001.2314558E-917.55000127
GTTGATA552.0610509E-417.18181824
TGCGTTG2250.015.627
ATCAACG1407.041308E-913.52
CTGCGTT2750.013.25454526
TATCAAC1451.1523298E-813.0344831
GTATCAA1051.17382315E-512.8571421
TCTGCGT5150.012.58252427
TCAACGC1552.9289367E-812.1935483
CAACGCA1604.558933E-811.81254
AACGCAG1852.8299837E-810.9459465
AATGGGT1050.002227673510.2857144
CTCTGCG6650.09.94736826
ACGTTCC1150.0047325059.3913047
ACTCTGC7500.08.8225
ACGCAGA2306.7984547E-78.8043486
Produced by FastQC (version 0.11.2)