FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_46.3520000005f43b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_46.3520000005f43b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57261
Sequences flagged as poor quality0
Sequence length33
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA2450.42786538830966975No Hit
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC2170.37896648678856465No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA1850.32308202790730167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.18337088070414417No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC860.1501894832433943No Hit
GTGAATGGGGAGTACGTTACTGCAAAAAAAAAA790.137964757863118No Hit
CCCCCCCTCCTTGCAGTAACGTACTCTGCGTTG750.13097920050296014No Hit
GTGAATGGGAGTACGTTACTGCAAAAAAAAAAA650.11351530710256544No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA650.11351530710256544No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA620.10827613908244704No Hit
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA620.10827613908244704No Hit
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.10827613908244704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGT200.00856906226.9834064
ACGTAGA257.7646325E-426.9834062
GTATCAT405.9439844E-726.9834061
CGTAGAG200.00856906226.9834063
CCTTTCA200.00856906226.98340612
TAGAGTG200.00856906226.9834065
TTCAGTA200.00856906226.98340615
TATCATC451.6600316E-623.9852522
AACGTAG300.002246639722.4861721
TAGCCTC300.002246639722.4861725
TGCGTTG6300.021.04222727
TCGCAGA559.45835E-619.6242967
ATCGCAG559.45835E-619.6242966
TCCCGCC350.005489694419.273863
ATCATCG602.0025933E-517.9889373
TACCTCC602.0025933E-517.9889376
TCATCGC602.0025933E-517.9889374
CATCGCA602.0025933E-517.9889375
CCCGCCT552.1119525E-417.1712594
TATCAAC2950.016.464451
Produced by FastQC (version 0.11.2)