FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_45.3520000005f411.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_45.3520000005f411.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7623
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT310.4066640430276794No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA270.3541912632821724No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA210.27548209366391185No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA210.27548209366391185No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA200.2623638987275351No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA180.23612750885478156No Hit
GTGAATGGGGTATCTCGACGCTAAAAAAAAAAA180.23612750885478156No Hit
GTGAATGGGATAGCGTCAAAAAAAAAAAAAAAA170.2230093139184048No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA160.20989111898202809No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA160.20989111898202809No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA150.19677292404565133No Hit
TATCAACGCAGAGTGAATGGGATAGCGTCGAGA150.19677292404565133No Hit
TATCAACGCAGAGTGAATGGGTAGCGTCGAGAT150.19677292404565133No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT140.18365472910927455No Hit
TATCAACGCAGAGTGAATGGGTAGCGTCGAAAA120.15741833923652104No Hit
TATCAACGCAGAGTGAATGGGGTAGCGTCAAAA120.15741833923652104No Hit
GTGAATGGGGTAGCGTCAAAAAAAAAAAAAAAA120.15741833923652104No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA110.1443001443001443No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.1443001443001443No Hit
GAGTGAATGGGTAGCGTCGAGATAAAAAAAAAA100.13118194936376756No Hit
GTGAATGGGTAGCGTCGAAAAAAAAAAAAAAAA100.13118194936376756No Hit
GTACGTATCTCGACGCTAAAAAAAAAAAAAAAA90.11806375442739078No Hit
GTGAATGGGTAGCGTCAAAAAAAAAAAAAAAAA90.11806375442739078No Hit
GTGAATGGGTAGCGTCGAGATAAAAAAAAAAAA90.11806375442739078No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA90.11806375442739078No Hit
ACGCAGAGTGAATGGGGTATCTCGACGCTAAAA90.11806375442739078No Hit
CCCTAGCGTCGAGATACGTACTCTGCGTTGATA80.10494555949101404No Hit
GAATGGGATAGCGTCAAAAAAAAAAAAAAAAAA80.10494555949101404No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC80.10494555949101404No Hit
GTGAATGGGTAGCGTCGAGAAAAAAAAAAAAAA80.10494555949101404No Hit
TATCAACGCAGAGTGAATGGGTAGCGTCAAAAA80.10494555949101404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT1950.016.00509527
TGCGTTG603.3900974E-415.83112527
TCAACGC851.9410816E-514.273223
TATCAAC851.9410816E-514.273221
CAACGCA851.9410816E-514.273224
ATCAACG903.3312805E-513.4802642
CTAGCGT1252.645047E-712.9410528
CTCTGCG2500.012.483973526
CTGCGTT800.002992112211.87334426
AACGCAG1051.416053E-411.554515
CAGAGTG1051.416053E-411.554519
AGAGTGA1102.1849856E-411.02930510
ACTCTGC2950.010.579638525
CGCAGAG1153.3024506E-410.549777
ATACGTA3100.010.43633319
GATACGT3150.010.27067718
AGATACG3150.010.27067717
GTACTCT3050.010.23276523
TACTCTG3050.010.23276524
ACGCAGA1204.897874E-410.1101976
Produced by FastQC (version 0.11.2)