FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_44.3520000005f3f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_44.3520000005f3f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34874
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA610.17491540976085337No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC590.1691804782932844No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGCAAA590.1691804782932844No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC580.1663130125594999No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA570.16344554682571544No Hit
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA430.1233010265527327No Hit
GAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA420.12043356081894822No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.11756609508516372No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC400.11469862935137926No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.10322876641624132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT1151.8189894E-1218.78061327
GTATCAA601.9094707E-518.0498811
TATCAAC1500.018.0498811
AGCCAGA450.001081915217.9980876
TCTGCGT8550.017.68233127
ATCAACG1650.016.3618972
ACCCAGA500.002190908216.1982787
TCAACGC1800.014.9984063
GCGTTGA1454.7293724E-1114.89496926
GCCCAGA550.00413531514.7257076
CCTCAGA550.00413531514.7257076
CAACGCA2000.014.1734944
TCAGAGT2750.013.2531378
CTCAGAG1356.6209395E-812.9986187
CTCTGCG11950.012.65137526
AACGCAG2250.012.5986615
ACTCTGC12700.011.90424625
GCCAGAG1954.0563464E-1011.767987
ACCAGAG2101.10958354E-1011.5701997
TACTCTG13200.011.5555924
Produced by FastQC (version 0.11.2)