FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_42.3520000005f3b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_42.3520000005f3b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51140
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC1750.342197888150176No Hit
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA1250.24442706296441144No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1090.21314039890496678No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA900.17598748533437622No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC880.17207665232694563No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG840.16425498631208446No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC760.14861165428236214No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA750.14665623777864686No Hit
GTATCAACGCAGAGTGAATGGGAACGTACTCTG620.12123582323034807No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA610.11928040672663277No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT610.11928040672663277No Hit
GAAACGTACTCTGCGTAAAAAAAAAAAAAAAAA590.1153695737192022No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAG570.1114587407117716No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA560.10950332420805631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCAG352.0026896E-423.142025
GTCAGTC552.0970494E-417.1811947
GATCTTG500.00219441816.2152791
ATCTTGG500.00220885516.1994132
CTTGGTG500.00220885516.1994134
GCCCAGT604.0718727E-415.7494296
GTATCAA1352.7102942E-1015.0141471
TATCAAC2250.014.4135821
ATCAACG2450.014.3260122
TCTGCGT10650.013.94315627
TCAACGC2550.013.7642073
CAACGCA2550.013.7642074
TCTTGGT600.00742388613.499513
AACGCAG3050.011.9503875
AACAGTC800.003571656311.8120727
TGCAGTC800.003571656311.8120728
ACAGTCT3300.011.4541318
CACAGTC1308.487565E-611.4226637
TGGAAAC13250.011.10525816
CTCTGGA13700.010.83902413
Produced by FastQC (version 0.11.2)