FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_40.3520000005f37f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_40.3520000005f37f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47777
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA5091.0653661803796806No Hit
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA4000.8372229315360947No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA3610.7555936957113255No Hit
GTCAAGCTCAACACGTACTCTGCGTTGATACCA3140.6572200012558345No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT2910.6090796826925089No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG2420.5065198735793374No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA2250.47093789898905336No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT2000.41861146576804736No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT1860.38930866316428403No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA1710.3579128032316805No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA1690.353726688574No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA1660.34744751658747935No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA1620.3390752872721184No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA1530.32023777131255626No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG1500.31395859932603554No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA1490.3118655419971953No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG1480.30977248466835505No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAACA1470.30767942733951487No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT1470.30767942733951487No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG1290.27000439542039056No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA1270.2658182807627101No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA1220.2553529941185089No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1210.2532599367896687No Hit
GGATGGATGCCCGGGCATTGAGAAGGAAGGACG1140.23860853548778702No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1100.23023630617242608No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA1060.2218640768570651No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA1040.21767796219938465No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA1010.21139879021286395No Hit
GAGATACCGTCTGTGATCCATGGATCTCCGATC1000.20930573288402368No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC1000.20930573288402368No Hit
GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA950.19884044623982253No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT930.19465433158214202No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA930.19465433158214202No Hit
GATACCGTCTGTGATCCATGGATCTCCGATCGG920.1925612742533018No Hit
GTGAATGGGGCGACCCCAGGTCAGGCGGGATTA880.18418904493794086No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA870.18209598760910062No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG840.17581681562257992No Hit
GCTCAACACGTACAAAAAAAAAAAAAAAAAAAA840.17581681562257992No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA840.17581681562257992No Hit
ACCGAGACCCCGTTAGTAGCGGCGAGCGAGAGC830.17372375829373968No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA820.17163070096489944No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA790.1653515289783787No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC780.16325847164953847No Hit
GAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCC770.16116541432069825No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG740.15488624233417753No Hit
CCGTATCCAAGCTCCGTGGCTAGTCTGCGCTCT740.15488624233417753No Hit
CATTGGATGGATGCCCGGGCATTGAGAAGGAAG740.15488624233417753No Hit
TATCAACGCAGAGTGAATGGGGAATGCATTGGA730.15279318500533728No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA730.15279318500533728No Hit
GTGAATGGGTCAAGCTCAACACGTACTCAAAAA700.14651401301881659No Hit
CTTTGAAAACTTAGCGAACTGAAACATCTAAGT700.14651401301881659No Hit
CCATGGATCTCCGATCGGGAAACCGTATCCAAG680.1423278983611361No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA670.14023484103229586No Hit
ATCCATGGATCTCCGATCGGGAAACCGTATCCA660.13814178370345562No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA660.13814178370345562No Hit
GTGATCCATGGATCTCCGATCGGGAAACCGTAT650.1360487263746154No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA640.13395566904577516No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA630.13186261171693495No Hit
GGCCATGGGGAGATACCGTCTGTGATCCATGGA620.1297695543880947No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA620.1297695543880947No Hit
CCCGTTAGTAGCGGCGAGCGAGAGCGGATTTGG620.1297695543880947No Hit
AACTGAAACATCTAAGTAGCTAAAGGAAGGGAA610.12767649705925446No Hit
GGTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAA610.12767649705925446No Hit
TAGTAGCGGCGAGCGAGAGCGGATTTGGGATTT600.12558343973041422No Hit
GGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT590.12349038240157398No Hit
ATGGATCTCCGATCGGGAAACCGTATCCAAGCT590.12349038240157398No Hit
CCCCGTTAGTAGCGGCGAGCGAGAGCGGATTTG580.12139732507273375No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACACGTAC570.11930426774389351No Hit
CCGATCGGGAAACCGTATCCAAGCTCCGTGGCT550.11511815308621304No Hit
GGTCAAGCTCAACACGTACTCTGCGTTGATACC540.1130250957573728No Hit
GAAACATCTAAGTAGCTAAAGGAAGGGAAATCA540.1130250957573728No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA540.1130250957573728No Hit
GTCTGTGATCCATGGATCTCCGATCGGGAAACC540.1130250957573728No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA530.11093203842853255No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAC530.11093203842853255No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAA520.10883898109969233No Hit
TATCAACGCAGAGTGAATGGGGTCAAGCTCAAC520.10883898109969233No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT510.10674592377085208No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA510.10674592377085208No Hit
CTGAAACATCTAAGTAGCTAAAGGAAGGGAAAT510.10674592377085208No Hit
CGCTTTCAGAGGCGAAAGGCCATGGGGAGATAC500.10465286644201184No Hit
GGGGAGATACCGTCTGTGATCCATGGATCTCCG500.10465286644201184No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTT500.10465286644201184No Hit
GCTCAACACGTACTAAAAAAAAAAAAAAAAAAA490.10255980911317161No Hit
GTGAATGGGTCAAGCTAAAAAAAAAAAAAAAAA490.10255980911317161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC455.4167685E-826.993692
TCGCAGA455.4167685E-826.993697
ATCATCG455.4167685E-826.993693
ATCGCAG455.4167685E-826.993696
TCATCGC455.4167685E-826.993694
CATCGCA455.4167685E-826.993695
GTATCAT501.5026853E-724.2943211
GCGTTGA1154.5656634E-1016.44823827
TATCAAC4300.015.3801271
ATCAACG4500.014.3966352
TGCGTTG2400.014.07401327
GTATCAA2650.013.7515031
TCAACGC4700.013.2096793
TCTGCGT6450.013.19684127
CAACGCA4750.013.070634
GGGCATT853.869526E-412.70291218
CCCGGGC853.869526E-412.70291215
CGGGCAT853.869526E-412.70291217
CCGGGCA853.869526E-412.70291216
ATATCAA750.00220095812.5970559
Produced by FastQC (version 0.11.2)