FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_4.3520000005eefe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_4.3520000005eefe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10143
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA1801.774622892635315No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA760.7492852213349108No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA620.6112589963521641No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA610.6013999802819678No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA500.4929508035098097No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA450.44365572315882873No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA360.354924578527063No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA350.3450655624568668No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA330.3253475303164744No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA320.3154885142462782No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA280.27605244996549344No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA280.27605244996549344No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA260.2563344178251011No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA260.2563344178251011No Hit
GTGAATGGGTATCGAAAAAAAAAAAAAAAAAAA250.24647540175490484No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA230.22675736961451248No Hit
GTATCGACGAAAAAAAAAAAAAAAAAAAAAAAA210.2070393374741201No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT210.2070393374741201No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA210.2070393374741201No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.1873213053337277No Hit
GAGTGAATGGGTATCGACGTAAAAAAAAAAAAA170.1676032731933353No Hit
GAGTGAATGGGTATCGACGAAAAAAAAAAAAAA170.1676032731933353No Hit
GTATCGACGTCTACGTACTCTTCGTTGATACCA170.1676032731933353No Hit
TCTACGTACTCAAAAAAAAAAAAAAAAAAAAAA170.1676032731933353No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA170.1676032731933353No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA160.1577442571231391No Hit
GTGAATGGGGTATCGACGAAAAAAAAAAAAAAA160.1577442571231391No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA150.14788524105294293No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA150.14788524105294293No Hit
GTATCGACGTCTACGTACTCAAAAAAAAAAAAA150.14788524105294293No Hit
GAGTGAATGGGTATCGACGTCAAAAAAAAAAAA140.13802622498274672No Hit
GTGAATGGGGTATCGACGTAAAAAAAAAAAAAA140.13802622498274672No Hit
GTGTATCGACGTCTACGTACTCTGCGTTGATAC140.13802622498274672No Hit
GTACGTAGACGTCGAAAAAAAAAAAAAAAAAAA140.13802622498274672No Hit
GAGTGAATGGGGTATCGACGTCAAAAAAAAAAA140.13802622498274672No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA130.12816720891255054No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA130.12816720891255054No Hit
AACGCAGAGTGAATGGGTATCGACGTAAAAAAA130.12816720891255054No Hit
CCGTATCGACGTCTACGTACTCTGCGTTGATAC130.12816720891255054No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA130.12816720891255054No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA130.12816720891255054No Hit
GGTATCGACGTCTACGTACTCTGCGTTGATACC120.11830819284235432No Hit
GCGTATCGACGTCTACGTACTCTGCGTTGATAC120.11830819284235432No Hit
ACGCAGAGTGAATGGGTATCGACAAAAAAAAAA120.11830819284235432No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA120.11830819284235432No Hit
CGTATCGACGTCTACGTACTCTGCGTTGATACC120.11830819284235432No Hit
GTGAATGGGGTATCGAAAAAAAAAAAAAAAAAA120.11830819284235432No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGTCA110.10844917677215814No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGAAA110.10844917677215814No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA110.10844917677215814No Hit
GTATCAACGCAGAGTGAATGGGGTATCGACGTC110.10844917677215814No Hit
AACGCAGAGTGAATGGGTATCGACGTCAAAAAA110.10844917677215814No Hit
GTATCGACGTCTACAAAAAAAAAAAAAAAAAAA110.10844917677215814No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA110.10844917677215814No Hit
GGTATCGACAAAAAAAAAAAAAAAAAAAAAAAA110.10844917677215814No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTAA110.10844917677215814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC351.754871E-423.32285726
GATACCA404.332578E-420.40750127
TATCAAC1100.019.5931591
GTTGATA500.001941985216.32624
TTGATAC500.001941985216.32625
ATCAACG1351.0913936E-1115.9647952
TCAACGC1401.8189894E-1115.3946273
CAACGCA1505.4569682E-1114.3683184
TCTGCGT2600.013.0817327
AACGCAG1652.5102054E-1013.0621065
TGGGTAT1051.5215692E-411.5459696
ACGCAGA1953.5415724E-911.0525516
CTCTGCG3100.010.97177426
CGCAGAG2005.2750693E-910.77623757
ATGGGTA1153.5447744E-410.5419715
AATGGGT1153.5447744E-410.5419714
GACGTCT3700.010.19373914
ACGTCTA3900.010.01637515
CGTATCG1351.3381803E-49.9779978
GTCTACG3800.09.92548217
Produced by FastQC (version 0.11.2)