FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_39.3520000005f356.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_39.3520000005f356.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34091
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1500.4399988266697956No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC920.26986594702414124No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA750.2199994133348978No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA670.196532809245842No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG650.19066615822357807No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA630.18479950720131413No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT570.1671995541345223No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT570.1671995541345223No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT500.1466662755565985No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT450.13199964800093866No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAA440.1290663224898067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.1290663224898067No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC440.1290663224898067No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTA380.11146636942301487No Hit
GGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGC380.11146636942301487No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTC370.1085330439118829No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA360.10559971840075093No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCC360.10559971840075093No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA360.10559971840075093No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG350.10266639288961897No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT350.10266639288961897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGC350.005442559719.2752117
TAAATTC500.002195846316.1911753
GTAAATT500.002195846316.1911752
TATCAAC1351.4551915E-1116.0148371
TCAACGC1351.4551915E-1115.9912843
ATCAACG1351.4551915E-1115.9912842
TGCGTTG2050.015.18274727
CAACGCA1508.185452E-1114.3921574
TCAGCTG600.007381469513.4926477
AACGCAG1706.002665E-1012.6989625
GTATCAA750.00215956312.6116831
TCTGCGT5850.011.7975227
GCAGCTG959.758974E-411.3622297
CTAGCTG959.758974E-411.3622297
CAGCTGA3250.010.7941188
CTCTGCG6600.010.66192926
AGAGTGA1701.3217432E-610.31790610
ACGCAGA2101.6603735E-810.2801126
CTGCGTT3050.010.20479826
ACTCTGC6900.010.19836725
Produced by FastQC (version 0.11.2)