FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_34.3520000005f2c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_34.3520000005f2c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29289
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA2500.8535627710061798No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA810.27655433780600225No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT710.24241182696575506No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA590.20144081395745841No Hit
GTGAATGGGATCGAGACAAAAAAAAAAAAAAAA530.18095530745331012No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA530.18095530745331012No Hit
GATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA520.17754105636928538No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA520.17754105636928538No Hit
TATCAACGCAGAGTGAATGGGATCGAGACAAAA510.1741268052852607No Hit
CATCGAGACGAGTACGTACTCTGCGTTGATACC510.1741268052852607No Hit
GAGTGAATGGGATCGAGACAAAAAAAAAAAAAA490.16729830311721125No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.16729830311721125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1638840520331865No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA460.1570555498651371No Hit
GACGAGTACAAAAAAAAAAAAAAAAAAAAAAAA430.14681279661306293No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGA420.1433985455290382No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA410.1399842944450135No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC410.1399842944450135No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA400.13657004336098877No Hit
TATCAACGCAGAGTGAATGGGACGAGTACGTAC400.13657004336098877No Hit
GAGTGAATGGGATCGAGACGAGAAAAAAAAAAA390.13315579227696406No Hit
GTGAATGGGATCGAGACGAGAAAAAAAAAAAAA360.12291303902488988No Hit
GTGAATGGGACGAGTACAAAAAAAAAAAAAAAA360.12291303902488988No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC360.12291303902488988No Hit
ACGCAGAGTGAATGGGATCGAGACGAGAAAAAA350.11949878794086519No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA340.11608453685684045No Hit
ACGCAGAGTGAATGGGATCGAGACAAAAAAAAA330.11267028577281574No Hit
GACGAGTACGTACTCAAAAAAAAAAAAAAAAAA330.11267028577281574No Hit
GAGTGAATGGGACGAGTACAAAAAAAAAAAAAA320.10925603468879101No Hit
TATCAACGCAGAGTGAATGGGACGAGTACAAAA310.1058417836047663No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAAA310.1058417836047663No Hit
GTGAATGGGATCGAGACGAAAAAAAAAAAAAAA300.10242753252074158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA2050.015.83801227
TATCAAC2150.013.7955871
GATACCA700.00126553713.52830127
TGATACC700.00126553713.52830126
GTATCAA1552.9676812E-812.1773371
ATCAACG2450.012.1063322
TCAACGC2600.011.407893
CAACGCA2800.011.0745424
TGCGTTG2950.011.00607726
TCTGCGT6100.010.64522127
AACGCAG3005.456968E-129.8868385
CTGCGTT3600.09.39465325
CTCTGCG7250.08.95666926
ACTCTGC7850.08.78908825
GCAGAGT4003.6379788E-128.4262828
TACTCTG8400.08.21361224
GTACTCT8650.08.13262123
CGCAGAG4053.45608E-117.98936377
ACGCAGA4104.5474735E-117.89193256
CAGAGTG3951.7644197E-107.85030849
Produced by FastQC (version 0.11.2)