FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_33.3520000005f2a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_33.3520000005f2a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34152
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATTCCGTTACGTACTCTGCGTTGATACCAC1020.2986647926914968No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA730.2137502928086203No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT730.2137502928086203No Hit
CCATTCCGTTACGTACTCTGCGTTGATACCACT620.18154134457718435No Hit
AGCCATTCCGTTACGTACTCTGCGTTGATACCA580.1698289997657531No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA490.1434762239400328No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC470.13762005153431717No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.13469196533145936No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC420.1229796205200281No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA420.1229796205200281No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA400.11712344811431248No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA390.11419536191145467No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA380.11126727570859686No Hit
GTACGTAACGAAAAAAAAAAAAAAAAAAAAAAA380.11126727570859686No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA370.10833918950573906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA2050.021.7564927
TGGGGAG404.9022847E-420.243416
TGCGTTG3050.014.62321526
ATGGGGA700.001296870213.4956085
TATCAAC1253.7302743E-712.9557842
TCAACGC1255.3507756E-611.8761354
ATCAACG1255.3507756E-611.8761353
CAACGCA1308.284864E-611.4193615
CTAGCCA959.7429956E-411.3647227
AACGCAG1452.2453223E-611.1687786
CTGCGTT4000.011.15020225
GTATCAA1351.2602055E-510.9964211
GTGAATG3000.010.3466331
TGAATGG2901.8189894E-1210.2380472
TCTGCGT6000.09.01026427
CCAGCCA1350.00173548628.9970727
AATGGGG1350.00173548628.9970724
CAGAGTG1556.182122E-48.70684410
ACGCAGA1752.1714708E-48.4829537
AGAGTGA1608.479867E-48.43475411
Produced by FastQC (version 0.11.2)