FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_31.3520000005f263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_31.3520000005f263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48855
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1190.2435779347047385No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA1110.22720294749769726No Hit
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA1060.2169685804932965No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA1040.21287483369153617No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA940.19240609968273462No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA940.19240609968273462No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA890.18217173267833384No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA760.15556237846689183No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA710.14532801146249105No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC660.13509364445809027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.13099989765632997No Hit
GTGAATGGGGAGTACGTCAGACATCGCAAAAAA530.10848429024664825No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA530.10848429024664825No Hit
GTTGCGATGTCTGACGTACTCTGCGTTGATACC510.10439054344488793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT200.00849792127.021561
TATCATC200.00854064626.9938472
ACCCTTG350.005465976419.2813197
GTTGATA1006.184564E-1118.91509227
TATCAAC2300.015.8604811
CGTTGAT1208.367351E-1015.76257826
ACTTGCG801.3550218E-515.1840397
GCGTTGA1201.4386387E-814.6366825
TCTGCGT5850.013.8572127
TCCTTGC600.007426835613.4969237
ATCAACG2600.012.9778112
GTATCAA1603.4633558E-912.6663571
TCAACGC2800.012.5328583
CAACGCA2950.011.8955944
GGTATCA800.003545307311.8219331
TGGGGAG800.003600312411.79770856
TGCGTTG2202.6193447E-1011.05427527
CTCTGCG8200.09.72117126
CTGCGTT2751.2369128E-99.33472126
AACGCAG3501.8189894E-129.25503355
Produced by FastQC (version 0.11.2)