FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_3.3520000005eed4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_3.3520000005eed4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20767
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTTCCTCACGTACTCTGCGTTGATACCA3461.6661048779313334No Hit
CTCTGTTCCTCACGTACTCTGCGTTGATACCAC920.44301054557711755No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA650.3129965811142678No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT580.27928925699426976No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA430.207059276737131No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA390.1877979486685607No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA390.1877979486685607No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA380.18298261665141813No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA350.1685366205999904No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA350.1685366205999904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.13482929647999228No Hit
GTGAATGGGACTCTGTTCAAAAAAAAAAAAAAA280.13482929647999228No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA270.1300139644628497No Hit
GTAGGATAGTGGCCTACCATGGTGGTAACGGGT240.11556796841142196No Hit
CCCCCACTCTGTTCCTCACGTACTCTGCGTTGA240.11556796841142196No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA240.11556796841142196No Hit
GTTCCTCACGTACTCAAAAAAAAAAAAAAAAAA230.11075263639427939No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA230.11075263639427939No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT220.1059373043771368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA659.822543E-1125.06491727
TGATACC659.822543E-1125.06491726
ACCACTC503.971183E-621.5651346
GCACTCT1002.3042958E-816.1738517
ATCAACG1002.3042958E-816.1738512
ACACTCT757.0340375E-616.173857
GTTGATA1106.7971996E-814.81108824
TCAACGC1107.442577E-814.7035013
TATCAAC1107.442577E-814.7035011
TTGATAC1151.1720658E-714.16712725
CAACGCA1151.2830242E-714.0642184
GCGTTGA2350.013.86569827
CGTTGAT1201.9719118E-713.5768323
CACTCTG4750.012.2013258
TCTGCGT8300.011.94106727
TGCGTTG4000.011.54030527
CCACTCT2005.5842975E-1011.4564777
AACGCAG1452.1390952E-611.1543795
CTCTGCG9200.010.7729226
CTGCGTT4550.010.44371426
Produced by FastQC (version 0.11.2)