FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_29.3520000005f220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_29.3520000005f220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49818
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGGACCTTACGTACTCTGCGTTGATACCAC4500.9032879682042635No Hit
TGCTTGGACCTTACGTACTCTGCGTTGATACCA4400.8832149022441689No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA3030.6082138985908708No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC1940.38941747962583806No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA1920.3854028664338191No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA1690.3392348147256012No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1350.27098639046127904No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA1260.25292063109719376No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA1100.22080372556104222No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA1090.2187964189650327No Hit
TATCAACGCAGAGTGAATGGGACCTTACGTACT1090.2187964189650327No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.2107671925809948No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA990.19872335300493796No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA970.19470873981291903No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA970.19470873981291903No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.17865028704484323No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT850.17062106066080535No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA830.1666064474687864No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA790.1585772210847485No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA750.1505479947007106No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA750.1505479947007106No Hit
TGCTTGGACCAAAAAAAAAAAAAAAAAAAAAAA640.12846762214460639No Hit
GTGAATGGGAGTACGTAAGGTCCAAAAAAAAAA630.12646031554859688No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA630.12646031554859688No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.12445300895258742No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA620.12445300895258742No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA620.12445300895258742No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA610.12244570235657795No Hit
GACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.12043839576056847No Hit
GGTCCAAGCAAAAAAAAAAAAAAAAAAAAAAAA600.12043839576056847No Hit
TACGTACTCTGCGTTGATACCAAAAAAAAAAAA550.11040186278052111No Hit
GAGTGAATGGGCCTTACGTACTCAAAAAAAAAA540.10839455618451162No Hit
GTGAATGGGTACGTAAGGTCCAAAAAAAAAAAA540.10839455618451162No Hit
GGTATCAACGCAGAGTGAATGGGCCTTACGTAC540.10839455618451162No Hit
TATCAACGCAGAGTGAATGGGGTCCAAAAAAAA530.10638724958850214No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCTGCGT520.10437994299249267No Hit
GGACCTTACGTACTCAAAAAAAAAAAAAAAAAA510.1023726363964832No Hit
GTATCATCGCAGAGTGAATGGGCCTTACGTACT510.1023726363964832No Hit
GAGTGAATGGGGTCCAAAAAAAAAAAAAAAAAA500.10036532980047372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG501.5048863E-724.2954712
TCTACGC501.5048863E-724.2954713
TACGCAG501.5048863E-724.2954715
CTACGCA501.5048863E-724.2954714
TATCTAC603.47718E-822.4958081
TTATGCT350.005466135719.2821224
TATCAAC3200.017.2936531
ATCAACG3500.016.1969812
TCAACGC3600.015.74706653
CAACGCA3700.015.6862664
ATGGGTG550.00417080514.72452817
TGGGGAG657.481187E-414.5357536
AACGCAG4200.014.1402235
ATACCAC1151.3564932E-714.11272827
TGGGTGC600.00742748113.49748518
GTTGCTT1007.333161E-613.4974847
GTATCAA1653.929017E-1013.0884711
ATGGGGA1152.1563064E-612.9106385
TGTGCTT1051.2129771E-512.8547487
TCTGCGT14200.012.66749927
Produced by FastQC (version 0.11.2)