FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_28.3520000005f1f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_28.3520000005f1f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19867
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA2321.167765641516082No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT1110.5587154577943323No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA860.43287864297578904No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT850.4278451703830473No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA690.34730960889917956No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA640.32214224593547086No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA620.3120753007499874No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA590.2969748829717622No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA570.28690793778627877No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC560.281874465193537No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA480.24160668445160316No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA470.23657321185886143No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA430.21643932148789452No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA430.21643932148789452No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA400.20133890370966928No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA390.19630543111692758No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA370.18623848593144413No Hit
CCCCCATCTCGTCCTCTACGTACTCTGCGTTGA370.18623848593144413No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA340.1711380681532189No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.16610459556047716No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA310.1560376503749937No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCAAAA280.1409372325967685No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCT270.13590376000402676No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA260.13087028741128504No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT250.1258368148185433No Hit
GTAGAGGACGAAAAAAAAAAAAAAAAAAAAAAA250.1258368148185433No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCA230.11576986963305984No Hit
GAGTGAATGGGATCTCGTCCTCAAAAAAAAAAA230.11576986963305984No Hit
GAGTGAATGGGATCTCGTCAAAAAAAAAAAAAA230.11576986963305984No Hit
GTGAATGGGGTACGTAGAGGACGAAAAAAAAAA220.11073639704031811No Hit
GTACGTAGAGGACGAAAAAAAAAAAAAAAAAAA210.10570292444757638No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA200.10066945185483464No Hit
GTCCTCTACGTACTCAAAAAAAAAAAAAAAAAA200.10066945185483464No Hit
GATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAA200.10066945185483464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC200.00825000827.106626
TACCACT200.00825000827.106627
TATCAAC1650.017.1625351
GATACCA653.627666E-516.68098327
ATCAACG1700.016.6577552
TCAACGC1750.016.181823
GCGTTGA2100.014.8440927
TGATACC751.2327604E-414.45685326
CAACGCA1900.014.1945764
GTTGATA853.5647827E-412.75604624
TTGATAC853.5647827E-412.75604625
AACGCAG2201.8189894E-1212.2589535
CGTTGAT959.093394E-411.41330523
GTATCAA1102.553457E-411.0330581
TGCGTTG3450.010.60693127
CGCAGAG2552.1827873E-1110.5763517
ACATCTC1050.002189526610.2741717
ACGCAGA2654.7293724E-1110.1516096
CAGAGTG2409.931682E-1010.1136369
AGAGTGA2409.931682E-1010.11363610
Produced by FastQC (version 0.11.2)