FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_27.3520000005f1de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_27.3520000005f1de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27431
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA650.23695818599394847No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT530.1932120593489118No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA460.16769348547264043No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA440.16040246436513433No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA420.15311144325762824No Hit
GTTTCGACCAAAAAAAAAAAAAAAAAAAAAAAA400.14582042215012214No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.14217491159636905No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA360.1312383799351099No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA350.12759286938135686No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC310.11301082716634464No Hit
CTCGTTGGTCGAAACGTACTCTGCGTTGATACC300.10936531661259159No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA290.10571980605883853No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTCG450.001073024618.0000025
TGCGTTG1500.017.09999827
TCTGCGT4700.016.08510827
CCCTCGT603.98475E-415.756
TATCAAC1600.015.18751
CTGCGTT1850.013.86486426
CTCTGCG5900.013.04237326
ATCAACG1801.0004442E-1012.7500012
CCTCGTT1501.8224455E-812.5999997
GTATCAA1101.8781428E-512.2727271
TTCGTTG1552.881643E-812.1935498
ACTCTGC6350.012.11811125
TCAACGC1953.8562575E-1011.7692313
TACTCTG6850.011.23357624
GTACTCT7050.011.10638223
CAACGCA2101.3205863E-910.9285724
CGTACTC7250.010.822
ACGTACT7200.010.68750121
TGGTCGA7400.010.39864813
AACGTAC7400.010.39864820
Produced by FastQC (version 0.11.2)