FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_23.3520000005f158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_23.3520000005f158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42315
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGATACGTACTCAAAAAAAAAAAAAAAAAAA7231.708613966678483No Hit
GATACGTACTCAAAAAAAAAAAAAAAAAAAAAA5281.2477844735909251No Hit
GTGAATGGGATAGCAGATACGTACTCAAAAAAA3760.8885737917995983No Hit
TATCAACGCAGAGTGAATGGGATAGCAGATACG3630.8578518255937612No Hit
GTGAATGGGATAGCAGATACGTACAAAAAAAAA2290.5411792508566702No Hit
GGATAGCAGATACGTACTCTGCGTTGATACCAC2040.48209854661467566No Hit
GCAGATACGTACAAAAAAAAAAAAAAAAAAAAA1930.456103036748198No Hit
GTATCAACGCAGAGTGAATGGGATAGCAGATAC1830.4324707550514002No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.41592815786364173No Hit
GGATAGCAGATACGTACTCAAAAAAAAAAAAAA1490.35212099728228763No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTC1330.3143093465674111No Hit
GATACGTACAAAAAAAAAAAAAAAAAAAAAAAA1270.3001299775493324No Hit
GATACGTACTAAAAAAAAAAAAAAAAAAAAAAA1230.29067706487061323No Hit
TATCAACGCAGAGTGAATGGGTAGCAGATACGT1210.2859506085312537No Hit
GAGTGAATGGGATAGCAGATACGTACTCAAAAA1150.271771239513175No Hit
GCAGATACGTACTAAAAAAAAAAAAAAAAAAAA1120.26468155500413565No Hit
GTATCATCGCAGAGTGAATGGGATAGCAGATAC1080.25522864232541653No Hit
GTGAATGGGATAGCAGATACGTACTAAAAAAAA1020.24104927330733783No Hit
TATCTACGCAGAGTGAATGGGATAGCAGATACG1010.23868604513765804No Hit
GGATAGCAGATACGTACAAAAAAAAAAAAAAAA990.23395958879829848No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACT910.21505376344086022No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA900.21269053527118043No Hit
ATCAACGCAGAGTGAATGGGATAGCAGATACGT870.20560085076214107No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACAA800.1890582535743826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.18196856906534326No Hit
GAGTGAATGGGATAGCAGATACGTACAAAAAAA750.1772421127259837No Hit
GTGAATGGGTAGCAGATACGTACTCAAAAAAAA740.1748788845563039No Hit
GGTATCAACGCAGAGTGAATGGGATAGCAGATA710.16778920004726455No Hit
GATAGCAGATACGTACTCTGCGTTGATACCACT680.1606995155382252No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC670.15833628736854544No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA600.14179369018078697No Hit
CAACTCAGAGTGAATGGGATAGCAGATACGTAC600.14179369018078697No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.13470400567174762No Hit
GAATGGGATAGCAGATACGTACTCAAAAAAAAA560.13234077750206782No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.12052463665366892No Hit
GTGAATGGGATAGCAGAAAAAAAAAAAAAAAAA500.11816140848398912No Hit
GATACGTACTCTAAAAAAAAAAAAAAAAAAAAA500.11816140848398912No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA470.11107172397494978No Hit
CAGATACGTACTCAAAAAAAAAAAAAAAAAAAA460.10870849580527No Hit
GTATCAACGCAGAGTGAATGGGTAGCAGATACG440.10398203946591043No Hit
ACGCAGAGTGAATGGGTAGCAGATACGTACTCA430.10161881129623065No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.10161881129623065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG405.8678415E-726.990512
CCGCCGG200.0085328726.990515
TCTACGC455.3920303E-826.990513
CTACGCA405.8678415E-726.990514
TACGCAG356.404529E-626.9905095
TATCTAC405.929214E-726.9585321
GTATCAT601.2769306E-924.7119871
CTCAGAG451.6389331E-623.9915664
TCAGAGT351.9988403E-423.1347225
TATCATC653.1814125E-922.8381232
TCATCGC653.1814125E-922.8381234
CATCGCA653.1814125E-922.8381235
CAACTCA553.7966674E-722.056981
ATCATCG707.485141E-921.206833
TCGCAGA707.588824E-921.1817047
ATCGCAG707.588824E-921.1817046
ACTCAGA559.339963E-619.6294633
GGTATCA559.436542E-619.6062051
AACTCAG651.866807E-618.6857382
GTATCAA1350.016.9738921
Produced by FastQC (version 0.11.2)