FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_21.3520000005f114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_21.3520000005f114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27550
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA920.33393829401088926No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT830.30127041742286753No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC760.27586206896551724No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA710.2577132486388385No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA670.24319419237749546No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA580.21052631578947367No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA570.20689655172413793No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.19600725952813067No Hit
GTGAATGGGTACGTACTCTGCGTTGATACAAAA460.16696914700544463No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA440.15970961887477314No Hit
GTACGTACTCTGCGTTGATACAAAAAAAAAAAA440.15970961887477314No Hit
TATCAACGCAGAGTGAATGGGCCAGTTCAAGGT410.14882032667876588No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA400.1451905626134301No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA390.14156079854809436No Hit
GGTACGTACTCTGCGTTGATACAAAAAAAAAAA390.14156079854809436No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA370.13430127041742287No Hit
GTGAATGGGCCAGTTCAAGGAAAAAAAAAAAAA360.13067150635208713No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA350.12704174228675136No Hit
TATCAACGCAGAGTGAATGGGTACGTACTCTGC330.11978221415607986No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA320.11615245009074411No Hit
GTATCAACGCAGAGTGAATGGGCCAGTTCAAGG320.11615245009074411No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA310.11252268602540835No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA310.11252268602540835No Hit
CTCCAGTTCAAGGTACGTACTCTGCGTTGATAC300.10889292196007261No Hit
ACCCAGTTCAAGGTACGTACTCTGCGTTGATAC290.10526315789473684No Hit
GCCCAGTTCAAGGTACGTACTCTGCGTTGATAC280.10163339382940109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCAG351.99218E-423.1038976
TCCCCAG300.002231049422.462126
TCTGCGT11100.018.83518227
GCCCCAG653.8975617E-516.5874126
CCGCCAG500.002198113816.1727286
TTCCAGT500.002198113816.1727287
ATCAACG1351.4551915E-1115.9730642
GTCCAGT707.3493014E-515.4025977
CTGCCAG801.3307856E-515.1619316
CTCTGCG14200.014.91448726
TCAACGC1454.7293724E-1114.8714743
TATCAAC1454.7293724E-1114.8714741
ACTCCAG550.004149044414.7024796
TGCCAGT1302.4992914E-914.5139867
TCCAGTT3200.013.8984378
ACTCTGC15400.013.75231925
TACTCTG15550.013.61965924
CACCAGT904.0455867E-513.4772737
CGCCAGT1202.2516906E-713.4772727
CCCCAGT2650.013.2229847
Produced by FastQC (version 0.11.2)