FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_2.3520000005eeba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_2.3520000005eeba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63859
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC2050.32101974662929267No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC1060.16599069825709767No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA880.1378035985530622No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT790.12371004870104449No Hit
CCCTAGCTCACTACCACGTACTCTGCGTTGATA790.12371004870104449No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG760.11901219875037192No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA710.11118244883258428No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT650.10178674893123914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCCA200.00859552526.9709579
GTTGATA900.022.58214827
ACGTAGC350.00551104919.2649696
CGCTAGC450.001102589217.9806396
ATGTAGC500.002232392616.1825736
GCTAGCT1108.284405E-814.71143157
GTCTACG657.573231E-414.5228221
TATCAAC1755.456968E-1213.8707781
ACGAGTC600.00750187613.4854795
CGAGTCG600.00750187613.4854796
GGGTTGT700.001326208613.48547812
TCTGCGT9750.013.06290527
CACTAGC853.929769E-412.6922156
CGGGTTG750.00222934212.58644711
TCTACGA750.002242553312.5765752
TGCAGCC906.1119866E-412.04381326
GTTGTTT906.360206E-411.98709214
GCAGCCC800.0034905711.85562827
ATCAACG2058.185452E-1111.8316212
TCGGGTT800.003616360211.79979310
Produced by FastQC (version 0.11.2)