FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_18.3520000005f0c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_18.3520000005f0c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17648
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGGTATCCAAAAAAAAAAAAAAAAAA2401.3599274705349047No Hit
GTGAATGGGTATCCAAAAAAAAAAAAAAAAAAA1700.9632819582955575No Hit
GTATCCAATGCACGTACTCTGCGTTGATACCAC1150.651631912964642No Hit
GGTATCCAATGCACGTACTCTGCGTTGATACCA1000.5666364460562103No Hit
TATCAACGCAGAGTGAATGGGTATCCAATGCAC860.48730734360834094No Hit
GTGAATGGGTATCCAATGCACAAAAAAAAAAAA790.4476427923844062No Hit
CCGGTATCCAATGCACGTACTCTGCGTTGATAC770.43631006346328194No Hit
TATCAACGCAGAGTGAATGGGGTATCCAAAAAA640.3626473254759746No Hit
CCAATGCACGTACTCAAAAAAAAAAAAAAAAAA580.328649138712602No Hit
GTGAATGGGTATCCAATGCAAAAAAAAAAAAAA550.3116500453309157No Hit
TATCAACGCAGAGTGAATGGGGTATCCAATGCA540.3059836808703536No Hit
GTGAATGGGTATCCAATGCACGTACTCAAAAAA540.3059836808703536No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA460.2606527651858567No Hit
CTGGTATCCAATGCACGTACTCTGCGTTGATAC450.25498640072529466No Hit
TATCAACGCAGAGTGAATGGGTATCCAAAAAAA440.24932003626473254No Hit
ACGCAGAGTGAATGGGGTATCCAAAAAAAAAAA420.23798730734360835No Hit
GAGTGAATGGGGTATCCAAAAAAAAAAAAAAAA420.23798730734360835No Hit
ACGCAGAGTGAATGGGTATCCAAAAAAAAAAAA380.21532184950135994No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA380.21532184950135994No Hit
GTATCAACGCAGAGTGAATGGGTATCCAATGCA370.20965548504079784No Hit
GTGAATGGGTATCCAATGCACGTACAAAAAAAA360.20398912058023572No Hit
GCGGTATCCAATGCACGTACTCTGCGTTGATAC360.20398912058023572No Hit
ACGGTATCCAATGCACGTACTCTGCGTTGATAC360.20398912058023572No Hit
GTGAATGGGGTATCCAATGCACGTACTCAAAAA320.1813236627379873No Hit
GTGAATGGGTATCCAATGAAAAAAAAAAAAAAA320.1813236627379873No Hit
GTGAATGGGGGTATCCAAAAAAAAAAAAAAAAA320.1813236627379873No Hit
GGGTATCCAATGCACGTACTCTGCGTTGATACC310.1756572982774252No Hit
GTGAATGGGTATCCAATGCACGTAAAAAAAAAA300.1699909338168631No Hit
GTATCAACGCAGAGTGAATGGGGTATCCAAAAA300.1699909338168631No Hit
GTGGTATCCAATGCACGTACTCTGCGTTGATAC290.164324569356301No Hit
CAGGTATCCAATGCACGTACTCTGCGTTGATAC280.1586582048957389No Hit
ATCCAATGCACGTACTCAAAAAAAAAAAAAAAA260.14732547597461468No Hit
GTGAATGGGTATCCAATAAAAAAAAAAAAAAAA260.14732547597461468No Hit
ACGCAGAGTGAATGGGGTATCCAATGCACGTAC250.14165911151405258No Hit
ATGGTATCCAATGCACGTACTCTGCGTTGATAC250.14165911151405258No Hit
CGGGTATCCAATGCACGTACTCTGCGTTGATAC250.14165911151405258No Hit
GGGGTATCCAATGCACGTACTCTGCGTTGATAC240.1359927470534905No Hit
GGTATCAACGCAGAGTGAATGGGTATCCAATGC240.1359927470534905No Hit
GTGAATGGGTATCCAATGCACGAAAAAAAAAAA240.1359927470534905No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT240.1359927470534905No Hit
GAGTGAATGGGTATCCAAAAAAAAAAAAAAAAA230.13032638259292836No Hit
ATCCAATGCACGTACAAAAAAAAAAAAAAAAAA230.13032638259292836No Hit
TCGGTATCCAATGCACGTACTCTGCGTTGATAC230.13032638259292836No Hit
TATCAACGCAGAGTGAATGGGGGTATCCAAAAA220.12466001813236627No Hit
GTGAATGGGGTATCCAATGCACGTACTCTGCGT220.12466001813236627No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG210.11899365367180417No Hit
GTGAATGGGGTATCCAATGCACGTAAAAAAAAA210.11899365367180417No Hit
TATCAACGCAGAGTGAATGGGTATCCAATGAAA200.11332728921124206No Hit
GTGAATGGGAATCCAAAAAAAAAAAAAAAAAAA200.11332728921124206No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT200.11332728921124206No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA180.10199456029011786No Hit
ACGCAGAGTGAATGGGTATCCAATGCACGTACT180.10199456029011786No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG180.10199456029011786No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA180.10199456029011786No Hit
GTGAATGGGGTATCCAATGCACAAAAAAAAAAA180.10199456029011786No Hit
GTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA180.10199456029011786No Hit
CAATGCACGTACTCAAAAAAAAAAAAAAAAAAA180.10199456029011786No Hit
GTGAATGGGGTATCAAAAAAAAAAAAAAAAAAA180.10199456029011786No Hit
GTATCAACGCAGAGTGAATGGGTATCCAAAAAA180.10199456029011786No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA180.10199456029011786No Hit
CCAATGCACGTACAAAAAAAAAAAAAAAAAAAA180.10199456029011786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTA200.00850324626.9232966
GCGGTAT401.7613247E-523.5578827
AGGGTAT351.9679459E-423.0771127
CCTGGTA350.005411987719.2309276
TCTGCGT4600.019.13058927
CTGGTAT653.8071295E-516.5681827
GCGTTGA1002.1142114E-816.24628424
CGTTGAT1002.1142114E-816.24628425
TCGGTAT500.002172578816.1539767
TATCAAC1550.015.6774191
GTTGATA1053.857349E-815.47265226
TGCGTTG1053.857349E-815.47265223
TTGATAC1053.857349E-815.47265227
CTCTGCG5850.015.04285726
GTATCAA902.2426266E-615.0000011
CGGTATC2650.013.715648
ACTCTGC6500.013.53857125
TACTCTG6750.013.23771524
GTACTCT6800.013.14037823
CCGGTAT1151.9978197E-612.8763587
Produced by FastQC (version 0.11.2)