FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_15.3520000005f065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_15.3520000005f065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30793
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC2140.6949631409735979No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA2050.6657357191569513No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA1540.5001136621959537No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1210.39294644886824925No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1130.3669665183645634No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT1010.3279966226090345No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA990.32150163998311304No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT900.2922742181664664No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA880.28577923554054496No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA870.2825317442275842No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT860.27928425291462344No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG850.27603676160166274No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA680.22082940928133016No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA660.21433442665540872No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT640.2078394440294872No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG640.2078394440294872No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA600.19484947877764427No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC600.19484947877764427No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA590.19160198746468354No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG560.18185951352580132No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA550.17861202221284056No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA550.17861202221284056No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA520.16886954827395836No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA510.16562205696099763No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT500.1623745656480369No Hit
GGATGGATGCCCGGGCATTGAGAAGGAAGGACG500.1623745656480369No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT490.15912707433507614No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA490.15912707433507614No Hit
GCCCTCAGCTATGCCAACGTACTCTGCGTTGAT450.1461371090832332No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG450.1461371090832332No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC450.1461371090832332No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT450.1461371090832332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.14288961777027248No Hit
TATCAACGCAGAGTGAATGGGGAATGCATTGGA420.136394635144351No Hit
GAGATACCGTCTGTGATCCATGGATCTCCGATC400.1298996525184295No Hit
GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA390.1266521612054688No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA390.1266521612054688No Hit
GTGAATGGGTGTGAGCAACTCTAACTATGCCAA380.12340466989250803No Hit
CCCCCTCAGCTATGCCAACGTACTCTGCGTTGA370.1201571785795473No Hit
ACTCTAACTATGCCAACGTACTCAAAAAAAAAA370.1201571785795473No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA370.1201571785795473No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA360.11690968726658657No Hit
GCTATGCCAACGTACTCAAAAAAAAAAAAAAAA340.11041470464066508No Hit
CCTCTCAGCTATGCCAACGTACTCTGCGTTGAT330.10716721332770436No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA320.1039197220147436No Hit
GGCCATGGGGAGATACCGTCTGTGATCCATGGA320.1039197220147436No Hit
CCCGTCAGCTATGCCAACGTACTCTGCGTTGAT320.1039197220147436No Hit
CATTGGATGGATGCCCGGGCATTGAGAAGGAAG310.10067223070178287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCAG200.00851278626.9804556
GATACCA551.2985765E-824.60784126
ATACCAC551.2985765E-824.60784127
TGATACC603.2714524E-822.55718825
TGAGAAG608.275074E-720.3014724
TTGATAC701.6685772E-719.33473424
GAAGGAA701.6685772E-719.33473427
GGAAGGG350.005436348319.27175317
ATTGGAT509.992086E-518.8863183
TGGATGG509.992086E-518.8863185
TTGGATG509.992086E-518.8863184
GAGAAGG651.782737E-618.73981925
GTTGATA753.447767E-718.04575223
TTGAGAA601.9010213E-518.04575223
ATTGAGA601.9010213E-518.04575222
CATTGAG601.955507E-517.9869721
ATCTAAG450.001082384517.986972
CATCTAA450.001082384517.986971
CTAAGTA450.001082384517.986974
TCTAAGT450.001082384517.986973
Produced by FastQC (version 0.11.2)