FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_12.3520000005f008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_12.3520000005f008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34451
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3551.0304490435691271No Hit
GTGAATGGGCGTAAGGATAAAAAAAAAAAAAAA2630.763403094249804No Hit
GCGTAAGGATTCACGTACTCTGCGTTGATACCA2320.6734202200226408No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA2270.6589068532118081No Hit
GGATTCACGTACTCAAAAAAAAAAAAAAAAAAA2130.6182694261414763No Hit
GTGAATGGGCGTAAGGAAAAAAAAAAAAAAAAA2120.6153667527793097No Hit
CACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA1810.5253838785521465No Hit
GCGTAAGGATAAAAAAAAAAAAAAAAAAAAAAA1790.5195785318278134No Hit
GTGAATGGGGCGTAAGGATAAAAAAAAAAAAAA1780.5166758584656469No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATAAA1530.444109024411483No Hit
ACGCAGAGTGAATGGGCGTAAGGATAAAAAAAA1450.4208876375141505No Hit
TATCAACGCAGAGTGAATGGGATTCACGTACTC1390.40347159734115123No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGAAAAA1290.37444486371948565No Hit
GAGTGAATGGGCGTAAGGATAAAAAAAAAAAAA1270.3686395169951525No Hit
GTGAATGGGGCGTAAGGAAAAAAAAAAAAAAAA1180.34251545673565353No Hit
ACGCAGAGTGAATGGGCGTAAGGAAAAAAAAAA1080.31348872311398795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.2728512960436562No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.26414327595715653No Hit
GAGTGAATGGGCGTAAGGAAAAAAAAAAAAAAA780.2264085222489913No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.2147978288003251No Hit
AACGCAGAGTGAATGGGCGTAAGGATAAAAAAA740.2147978288003251No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGATAA720.208992482075992No Hit
GGCGTAAGGATAAAAAAAAAAAAAAAAAAAAAA710.20608980871382543No Hit
GAGTGAATGGGGCGTAAGGATAAAAAAAAAAAA700.20318713535165886No Hit
GAGTGAATGGGGCGTAAGGAAAAAAAAAAAAAA690.20028446198949232No Hit
GGCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAA660.19157644190299267No Hit
GTATCAACGCAGAGTGAATGGGATTCACGTACT650.1886737685408261No Hit
GATTCACGTACTCAAAAAAAAAAAAAAAAAAAA630.182868421816493No Hit
ATTCACGTACTCTGCGAAAAAAAAAAAAAAAAA590.17125772836782677No Hit
GCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAAA590.17125772836782677No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGAAAA570.16545238164349366No Hit
CGTAAGGATTCACGTACTCTGCGTTGATACCAC570.16545238164349366No Hit
TATCTACGCAGAGTGAATGGGCGTAAGGATAAA550.15964703491916055No Hit
TATCAACGCAGAGTGAATGGGGCGTAAGGATAA530.15384168819482744No Hit
TTCACGTACTCTGCGAAAAAAAAAAAAAAAAAA520.15093901483266087No Hit
ACGCAGAGTGAATGGGGCGTAAGGAAAAAAAAA510.14803634147049433No Hit
GAATGGGGCGTAAGGAAAAAAAAAAAAAAAAAA490.14223099474616122No Hit
GAATGGGCGTAAGGATAAAAAAAAAAAAAAAAA480.13932832138399465No Hit
AACGCAGAGTGAATGGGCGTAAGGAAAAAAAAA460.13352297465966156No Hit
GTATCATCGCAGAGTGAATGGGCGTAAGGATAA430.12481495457316188No Hit
TATCAACGCAGAGTGAATGGGGCGTAAGGAAAA410.11900960784882877No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATTCA400.1161069344866622No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1161069344866622No Hit
GGATTCACGTACTCTGCGAAAAAAAAAAAAAAA390.11320426112449565No Hit
GTGAATGGGCGTAAGGATTCACGTACTCAAAAA390.11320426112449565No Hit
GTGAATGGGGGCGTAAGGAAAAAAAAAAAAAAA390.11320426112449565No Hit
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAC380.11030158776232911No Hit
GAATGGGCGTAAGGAAAAAAAAAAAAAAAAAAA370.10739891440016255No Hit
GTGAATGGGGGCGTAAGGATAAAAAAAAAAAAA360.104496241037996No Hit
GCGTAAGGATTCACGTACTCAAAAAAAAAAAAA360.104496241037996No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.104496241037996No Hit
GTGAATGGGAGCGTAAGGATAAAAAAAAAAAAA360.104496241037996No Hit
GAGTGAATGGGATTCACGTACTCTGCGAAAAAA360.104496241037996No Hit
AACGCAGAGTGAATGGGATTCACGTACTCTGCG350.10159356767582943No Hit
GTATCATCGCAGAGTGAATGGGCGTAAGGAAAA350.10159356767582943No Hit
GTGAATGGGAGCGTAAGGAAAAAAAAAAAAAAA350.10159356767582943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG200.00851005626.9912661
ATCTACG200.00851005626.9912662
GTATCAT455.329275E-826.9912661
TATCTAC200.00851005626.9912661
TATCATC455.329275E-826.9912662
CTACGCA200.00851005626.9912664
CATCGCA451.6237591E-623.9922375
ATCATCG504.0559007E-621.5930143
TCGCAGA454.4491244E-520.9932067
ATCGCAG454.4491244E-520.9932066
GATACCA559.138563E-619.65862827
TGATACC559.138563E-619.65862826
TCATCGC559.254945E-619.6300134
TATCAAC3300.017.9941771
GTTGATA653.8494072E-516.63422224
TTGATAC653.8494072E-516.63422225
CGTTGAT653.8494072E-516.63422223
ATCAACG3500.016.5803492
TCTGCGT6900.015.86579427
GGTATCA604.0321966E-415.7449051
Produced by FastQC (version 0.11.2)