FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_10.3520000005efb9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_10.3520000005efb9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30250
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGAGACGTACGTACTCTGCGTTGATACCA4061.3421487603305784No Hit
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA2900.9586776859504132No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT1740.5752066115702479No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA1700.5619834710743801No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA1650.5454545454545455No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA1460.4826446280991736No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA1130.37355371900826445No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA730.2413223140495868No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA630.20826446280991737No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA620.2049586776859504No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA620.2049586776859504No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA530.17520661157024794No Hit
GTATCAACGCAGAGTGAATGGGGAGACGTACGT510.16859504132231404No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT480.1586776859504132No Hit
TATCAACGCAGAGTGAATGGGAGAGACGTACGT440.14545454545454545No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACAA390.12892561983471076No Hit
ATGCAGAGACGTACGTACTCTTCGTTGATACCA390.12892561983471076No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA390.12892561983471076No Hit
ATGCAGAGACAAAAAAAAAAAAAAAAAAAAAAA380.1256198347107438No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.11900826446280992No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA360.11900826446280992No Hit
CCCCCATGCAGAGACGTACGTACTCTGCGTTGA350.11570247933884298No Hit
GTGAATGGGATGCAGAGACAAAAAAAAAAAAAA350.11570247933884298No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA340.11239669421487604No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA310.1024793388429752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC200.0084275427.03476726
TACCACT200.0084275427.03476727
GTATCAA1204.3655746E-1116.8688031
TGATACC603.9630829E-415.77028126
GCGTTGA2150.015.08917327
GATACCA657.279541E-414.55718327
TATCAAC1952.910383E-1112.4569611
TCAACGC2202.0008883E-1111.6548093
ATCAACG2202.0008883E-1111.6548092
TTGATAC850.005471400511.13196425
GGGGAGA1102.6222295E-411.0413987
CAACGCA2409.640644E-1110.6835754
GTTGATA900.00836928410.5135224
TGGGGAG1050.002234302710.2819376
TGCGTTG3400.09.93925326
AATGGGG2254.7326466E-89.5964754
AACGCAG2553.1759555E-99.5259125
TGAATGG5150.09.1713872
CTGCGTT3700.09.13336825
ATGGGGA1504.4198788E-48.9966955
Produced by FastQC (version 0.11.2)