FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_95.3520000005ee5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_95.3520000005ee5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA50.49019607843137253No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA50.49019607843137253No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA50.49019607843137253No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA40.39215686274509803No Hit
GTGAATGGGGAGTACGTTAGCAAAAAAAAAAAA30.29411764705882354No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GATCCAGTCTGCTAACGTACTCTGCGTTGATAC30.29411764705882354No Hit
GAGTGAATGGGCTCCAGTCAAAAAAAAAAAAAA30.29411764705882354No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC30.29411764705882354No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
CTTCCAGTCTGCTAACGTACTCTGCGTTGATAC30.29411764705882354No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA30.29411764705882354No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
ACGCAGAGTGAATGGGATCCAGTCAAAAAAAAA30.29411764705882354No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT20.19607843137254902No Hit
GAGTGAATGGGCTCCAGTAAAAAAAAAAAAAAA20.19607843137254902No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA20.19607843137254902No Hit
TCTCCAGTCTGCTAACGTACTCTGCGTTGATAC20.19607843137254902No Hit
AGAGTTTGATTTTTCTTATTAAAAACCCGTAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA20.19607843137254902No Hit
ATCAACGCAGAGTGAATGGGTCCAGTCTGAAAA20.19607843137254902No Hit
GGGGACTGGATTTAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGAGTACGTTAGCAAAAAAAAAAAAA20.19607843137254902No Hit
GAGTGAATGGGTCCAGTCTGAAAAAAAAAAAAA20.19607843137254902No Hit
GTATCAACGCAGAGTGAATGGGGTCCAGTCTAA20.19607843137254902No Hit
GAGTGAATGGGATAATCCAGTCAAAAAAAAAAA20.19607843137254902No Hit
ACATCCAGTCTGCTAACGTACTCTGCGTTGATA20.19607843137254902No Hit
ACGCAGAGTGAATGGGTCCAGTCTGAAAAAAAA20.19607843137254902No Hit
GTGAATGGGAAAGTCCAGTAAAAAAAAAAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGACCGTCCAAAAAAAAAA20.19607843137254902No Hit
CCAGTCTGCTAACGTACTCTGCGTTGATACCAC20.19607843137254902No Hit
GCTCCAGTCTGCTAACGTACTCTGCGTTGATAC20.19607843137254902No Hit
GTATCATCGCAGAGTGAATGGGCGAGTCCAAAA20.19607843137254902No Hit
GTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTTTAGCAGACTGAGACAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAGTGAATGGGGAGTACGTTAGCAAAAAAAAAA20.19607843137254902No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA20.19607843137254902No Hit
GTCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACTCCCAATTCCAGTCTGCTAACGTACTCTGCG20.19607843137254902No Hit
GAGTGAATGGGGCACTCCAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACGCAAAGTGAATGGGGAGTTCCAGTCAAAAAA20.19607843137254902No Hit
GTGAATGGGTCCAGTCAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GGTATCAACGCAGAGTGAATGGGAGCTTCCAGT20.19607843137254902No Hit
ACGCAGAGTGAATGGGAGACTGGAAAAAAAAAA20.19607843137254902No Hit
ACTGGGATGTCCAGTCTGCTAACGTACTCTGCG20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)