FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_80.3520000005ec86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_80.3520000005ec86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65665
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC10361.5777050178938552No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA9601.4619660397472016No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA8561.303586385441255No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA6901.0507880910683012No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA6450.9822584329551511No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA5300.8071270844437676No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA4700.7157542069595675No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA4540.6913881062971141No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA3960.6030609913957207No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA3590.5467143836137973No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA3500.5330084519911673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3490.531485570699764No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA3340.5086423513287139No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA3100.4720932003350339No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT3050.46447879387801716No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA2920.44468133708977386No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC2840.4324982867585472No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA2830.4309754054671438No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA2820.42945252417574054No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC2750.41879235513591717No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.37006015381101043No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA2390.3639686286453971No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA2370.36092286606259044No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA2330.3548313408969771No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA2290.34873981573136376No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC2270.34569405314855706No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.309144902154877No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA1920.29239320794944035No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA1900.28934744536663365No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA1770.26954998857839035No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA1740.2649813447041803No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA1710.2604127008299703No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA1700.25888981953856693No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA1670.25432117566435697No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA1630.24822965049874363No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG1620.24670676920734028No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA1590.24213812533313028No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA1520.23147795629330695No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC1460.22234066854488693No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA1430.21777202467067694No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1230.1873143988426102No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.1873143988426102No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC1220.18579151755120688No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA1220.18579151755120688No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA1190.18122287367699688No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA1180.17969999238559353No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA1180.17969999238559353No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA1140.17360846721998022No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA1140.17360846721998022No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA1090.16599406076296352No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA1080.1644711794715602No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA1050.15990253559735018No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.15990253559735018No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA1010.15381101043173684No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA1010.15381101043173684No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC1010.15381101043173684No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA980.14924236655752685No Hit
GTGAATGGGCTAACTATCAAAAAAAAAAAAAAA960.14619660397472017No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA950.14467372268331682No Hit
GTGAATGGGGCTAACAAAAAAAAAAAAAAAAAA950.14467372268331682No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA910.1385821975177035No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA910.1385821975177035No Hit
ACGCAGAGTGAATGGGGCTAACTAAAAAAAAAA910.1385821975177035No Hit
CTAACTATCCACAAAAAAAAAAAAAAAAAAAAA890.13553643493489684No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA890.13553643493489684No Hit
TATCAACGCAGAGTGAATGGGCTAACTATAAAA890.13553643493489684No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA880.1340135536434935No Hit
CTAACTATCCACGTACTCTGCGTTGATACCACT880.1340135536434935No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA870.13249067235209014No Hit
ATCAACGCAGAGTGAATGGGGCTAACTATCCAC870.13249067235209014No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA860.13096779106068682No Hit
AACTATCCACGTACAAAAAAAAAAAAAAAAAAA860.13096779106068682No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA840.12792202847788015No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA830.1263991471864768No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA800.1218305033122668No Hit
CGGCTAACTATCCACGTACTCTGCGTTGATACC790.12030762202086347No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.11878474072946013No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA770.11726185943805681No Hit
GCTAACTATCCACGTACTCAAAAAAAAAAAAAA760.11573897814665347No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC740.1126932155638468No Hit
GAGTGAATGGGCTAACTAAAAAAAAAAAAAAAA740.1126932155638468No Hit
GTGAATGGGGCTAACTATAAAAAAAAAAAAAAA730.11117033427244347No Hit
CCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.11117033427244347No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.10507880910683014No Hit
GGGCTAACTATCCACGTACTCAAAAAAAAAAAA680.1035559278154268No Hit
TATCAACGCAGAGTGAATGGGGCTAACTAAAAA680.1035559278154268No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA670.10203304652402344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTG200.00846179727.05886827
CTTCGTT200.00846179727.05886826
TCTTCGT200.00846179727.05886825
AACGTAG501.5080332E-724.3158021
GTAGAGT501.5305159E-724.2787364
CGTAGAG501.5305159E-724.2787363
TAGAGTG501.5305159E-724.2787365
GTATCAT451.6479244E-624.0156081
TATCTAC352.001789E-423.1579061
ATCTACG352.0226323E-423.1226042
TCTACGC352.0226323E-423.1226043
ACGTAGA553.839541E-722.0715772
TACGCAG404.987712E-420.2322795
CTACGCA404.987712E-420.2322794
TCGCAGA559.514455E-619.6191797
ATCATCG559.514455E-619.6191793
ATCGCAG559.514455E-619.6191796
TATCATC602.014392E-517.9842472
TCATCGC602.014392E-517.9842474
GGTATCA1300.017.6653251
Produced by FastQC (version 0.11.2)