FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_79.3520000005ec50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_79.3520000005ec50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63800
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA3010.47178683385579934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2930.45924764890282127No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA2630.4122257053291536No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG2610.4090909090909091No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA2370.3714733542319749No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA2290.3589341692789969No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT2190.3432601880877743No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA2110.33072100313479624No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA2060.3228840125391849No Hit
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA1920.3009404388714733No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT1920.3009404388714733No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1840.2884012539184953No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA1760.27586206896551724No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA1750.274294670846395No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT1600.25078369905956116No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA1570.24608150470219436No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA1560.24451410658307213No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA1530.23981191222570533No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA1470.23040752351097177No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA1400.219435736677116No Hit
CCCCGTTAGTAGCGGCGAGCGAGAGCGGATTTG1350.2115987460815047No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA1270.19905956112852663No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA1200.18808777429467086No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT1200.18808777429467086No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA1200.18808777429467086No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA1120.1755485893416928No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG1070.1677115987460815No Hit
TAGTAGCGGCGAGCGAGAGCGGATTTGGGATTT990.15517241379310345No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA980.1536050156739812No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA970.15203761755485892No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA970.15203761755485892No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA940.14733542319749215No Hit
CCCGTTAGTAGCGGCGAGCGAGAGCGGATTTGG940.14733542319749215No Hit
ACCGAGACCCCGTTAGTAGCGGCGAGCGAGAGC920.14420062695924765No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG910.1426332288401254No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA900.14106583072100312No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG890.13949843260188086No Hit
CTTTGAAAACTTAGCGAACTGAAACATCTAAGT880.13793103448275862No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA880.13793103448275862No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA860.13479623824451412No Hit
GAGTGAATGGGGACATCGCAAAAAAAAAAAAAA840.1316614420062696No Hit
GTGAATGGGTTGCGATGAAAAAAAAAAAAAAAA840.1316614420062696No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC830.13009404388714735No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG810.12695924764890282No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA800.12539184952978058No Hit
GTATCAACGCAGAGTGAATGGGATGTCTGACGT770.1206896551724138No Hit
CTTGCGATGTCTGACGTACTCTGCGTTGATACC760.11912225705329155No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA760.11912225705329155No Hit
ATCAACGCAGAGTGAATGGGATGTCTGACGTAC750.11755485893416928No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA740.11598746081504703No Hit
AACGCAGAGTGAATGGGATGTCTGACGTACTCA730.11442006269592477No Hit
AACTGAAACATCTAAGTAGCTAAAGGAAGGGAA710.11128526645768025No Hit
CCGTTAGTAGCGGCGAGCGAGAGCGGATTTGGG700.109717868338558No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA690.10815047021943575No Hit
GAATGGGACATCGCAAAAAAAAAAAAAAAAAAA670.10501567398119123No Hit
GTTAGTAGCGGCGAGCGAGAGCGGATTTGGGAT660.10344827586206896No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT650.10188087774294671No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA650.10188087774294671No Hit
ATGTCTGACGTACTCAAAAAAAAAAAAAAAAAA640.10031347962382445No Hit
GTGAATGGGATTGCGATAAAAAAAAAAAAAAAA640.10031347962382445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAC200.00859674326.9701731
GTATGGG257.79664E-426.9701713
GGAGATA757.4802683E-616.1821022
GAGATAC801.3795563E-515.1707223
AGATACC801.3795563E-515.1707224
GGGAGAT852.4466277E-514.2783281
TATCAAC4400.013.7915661
GGATCTC904.038657E-513.53821924
ACCGTCT802.3541096E-413.4850868
CCGTCTG802.3541096E-413.4850869
TACCGTC802.3541096E-413.4850867
GTATCAA2850.013.2485071
AGTAGCT1451.2669261E-813.0200837
CTAAGTA1357.062772E-812.985644
ATCTCCG956.7089146E-512.82568126
ATGGATC956.7089146E-512.82568122
TGGATCT956.7089146E-512.82568123
TCTCCGA956.7089146E-512.82568127
GATACCG853.9307328E-412.6918475
AAGTAGC1502.035813E-812.5860816
Produced by FastQC (version 0.11.2)