FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_71.3520000005eb6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_71.3520000005eb6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73286
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGATACGTACTCAAAAAAAAAAAAAAAAAAA12761.7411238162814182No Hit
GATACGTACTCAAAAAAAAAAAAAAAAAAAAAA7270.9920039298092406No Hit
GTGAATGGGATAGCAGATACGTACTCAAAAAAA6400.8732909423355075No Hit
GGATAGCAGATACGTACTCTGCGTTGATACCAC6270.8555522200693175No Hit
TATCAACGCAGAGTGAATGGGATAGCAGATACG5520.7532134377643751No Hit
GTGAATGGGATAGCAGATACGTACAAAAAAAAA4880.6658843435308245No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4430.604481074147859No Hit
GCAGATACGTACAAAAAAAAAAAAAAAAAAAAA3350.4571132276287422No Hit
GTATCAACGCAGAGTGAATGGGATAGCAGATAC3100.42300030019376145No Hit
GGATAGCAGATACGTACTCAAAAAAAAAAAAAA2540.34658734273940456No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTC2540.34658734273940456No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.32066151788881914No Hit
GATACGTACAAAAAAAAAAAAAAAAAAAAAAAA2330.31793248369402066No Hit
GATAGCAGATACGTACTCTGCGTTGATACCACT2250.3070163469148268No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA2070.2824550391616407No Hit
GCAGATACGTACTAAAAAAAAAAAAAAAAAAAA2040.278361487869443No Hit
GAGTGAATGGGATAGCAGATACGTACTCAAAAA2030.27699697077204377No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1990.2715389023824468No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACT1890.25789373140845456No Hit
TATCAACGCAGAGTGAATGGGTAGCAGATACGT1840.2510711459214584No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACAA1780.242884043337063No Hit
GATACGTACTAAAAAAAAAAAAAAAAAAAAAAA1730.23606145785006685No Hit
GGATAGCAGATACGTACAAAAAAAAAAAAAAAA1510.2060420817072838No Hit
GTGAATGGGATAGCAGATACGTACTAAAAAAAA1450.1978549791228884No Hit
ATCAACGCAGAGTGAATGGGATAGCAGATACGT1410.1923969107332915No Hit
ACGCAGAGTGAATGGGTAGCAGATACGTACTCA1270.17329367136970228No Hit
GGTATCAACGCAGAGTGAATGGGATAGCAGATA1240.16920012007750457No Hit
CCGGATAGCAGATACGTACTCTGCGTTGATACC1220.16647108588270612No Hit
GAGTGAATGGGATAGCAGATACGTACAAAAAAA1200.16374205168790765No Hit
GTGAATGGGTAGCAGATACGTACTCAAAAAAAA1200.16374205168790765No Hit
GAATGGGATAGCAGATACGTACTCAAAAAAAAA1180.1610130174931092No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA1070.14600332942171765No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACA1050.1432742952269192No Hit
GGATAGCAGAAAAAAAAAAAAAAAAAAAAAAAA1030.14054526103212073No Hit
CAGATACGTACTCAAAAAAAAAAAAAAAAAAAA1020.1391807439347215No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT980.13372267554512457No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.13235815844772536No Hit
GTGAATGGGATAGCAGAAAAAAAAAAAAAAAAA950.12962912425292689No Hit
GTATCAACGCAGAGTGAATGGGTAGCAGATACG950.12962912425292689No Hit
CAACGCAGAGTGAATGGGATAGCAGATACGTAC900.12280653876593074No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC890.1214420216685315No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.11871298747373304No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA860.11734847037633382No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA850.11598395327893458No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA840.11461943618153536No Hit
GTGAATGGGATAGCAGATACGTAAAAAAAAAAA820.1118904019867369No Hit
GCGGATAGCAGATACGTACTCTGCGTTGATACC790.1077968506945392No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT780.10643233359713997No Hit
TAGCAGATACGTACAAAAAAAAAAAAAAAAAAA760.1037032994023415No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.1037032994023415No Hit
GTGAATGGGCGGATAGCAGAAAAAAAAAAAAAA750.10233878230494228No Hit
GATACGTACTCTAAAAAAAAAAAAAAAAAAAAA750.10233878230494228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAAAT257.767827E-426.9938452
TATCATC356.483524E-626.9938452
CTGAGTG257.7989715E-426.9753954
AATTCTG200.00859683626.9753935
ATCATCG307.112739E-526.9753913
TCATCGC307.112739E-526.9753914
CATCGCA307.112739E-526.9753915
AACGTAG501.515873E-724.3110891
TCGCAGA352.026236E-423.1217677
ATCGCAG352.026236E-423.1217676
ACGCTGA300.00223858122.5102671
TGAGTGA300.002256476622.4794945
GTATCAT454.511449E-521.0095831
ACGTAGA608.8386514E-720.2453822
CGTAGAG608.897041E-720.2315443
GTAGAGT559.54212E-619.6184674
TAGAGTG559.54212E-619.6184675
GTATCAA3050.019.4842971
GAGTAAA350.00546999719.2945161
GTAAATG350.00551348819.2681393
Produced by FastQC (version 0.11.2)