FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_7.3520000005e365.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_7.3520000005e365.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190630
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA12620.6620154225462939No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA12530.657294234905314No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA11430.5995908304044484No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA10550.553428106803756No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA10260.5382153910717096No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC9710.5093636888212768No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA9080.4763153753344174No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA8640.4532340135340713No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA7940.41651366521533856No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA7580.397628914651419No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA6670.34989246183706657No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA6320.33153228767770027No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA6130.32156533599118714No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA5730.3005822798090542No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA5400.28327125845879453No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG5190.27225515396317473No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA5050.2649110842994282No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA4930.2586161674447883No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA4920.258091591040235No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT4560.23920684047631538No Hit
GTGAATGGGGCGACCCCAGGTCAGGCGGGATTA4430.23238734721712215No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT4260.22346954833971566No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTT4260.22346954833971566No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA4000.20983056182132925No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC3950.20720767979856267No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA3950.20720767979856267No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTA3730.19566699889838954No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG3730.19566699889838954No Hit
GGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT3620.189896658448303No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA3510.18412631799821644No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA3330.17468394271625662No Hit
GGCGAGAGACCGATAGCGAACAAGTACCGCGAG3210.16838902586161675No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA3160.16576614383885013No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAAA2950.15475003934323034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2940.15422546293867703No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA2850.1495042752976971No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2800.1468813932749305No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA2780.14583224046582385No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA2740.14373393484761054No Hit
ATACGGGCGAGAGACCGATAGCGAACAAGTACC2700.14163562922939726No Hit
AACGCAGAGTGAATGGGCATTGCTGAAAAAAAA2700.14163562922939726No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA2690.14111105282484396No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAG2600.13638986518386403No Hit
GCTGAAGACGTAAAAAAAAAAAAAAAAAAAAAA2600.13638986518386403No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC2490.13061952473377747No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA2420.12694748990190421No Hit
GTCTTCAGCAAAAAAAAAAAAAAAAAAAAAAAA2400.12589833709279755No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2350.12327545507003096No Hit
CAAGTACCGCGAGGTAAAAAAAAAAAAAAAAAA2320.12170172585637098No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA2230.11698053821539106No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA2230.11698053821539106No Hit
GAGTGAATGGGGCGACCCCAGGTCAGGCGGGAT2220.11645596181083775No Hit
GGTATCAACGCAGAGTGAATGGGCATTGCTGAA2200.1154068090017311No Hit
ACGCAGAGTGAATGGGGCGACCCCAGGTCAGGC2130.11173477416985783No Hit
CCGCATTGCTGAAGACGTACTCTGCGTTGATAC2040.10701358652887792No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2040.10701358652887792No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAA2020.1059644337197713No Hit
GGCGGGATTACCCGCTGAGTTTAAGCATATCAA2020.1059644337197713No Hit
GCTGAAGACGTACTAAAAAAAAAAAAAAAAAAA2000.10491528091066463No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAG1990.10439070450611132No Hit
GAGTGAATGGGCATTGCTGAAGACAAAAAAAAA1990.10439070450611132No Hit
GCATTGCTGAAAAAAAAAAAAAAAAAAAAAAAA1960.10281697529245136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATGC200.00860569926.9915984
GATATCA200.00860569926.9915981
GTATCTA200.00860569926.9915981
CCTCACA200.00860569926.9915981
TTAATTT200.00860569926.99159815
CTCACAT200.00860569926.9915982
GTATCAT651.2369128E-1024.915321
TCGCAGA703.1468517E-1023.1356567
ATCGCAG703.1468517E-1023.1356566
TCATCGC806.91216E-1121.9306744
GGCCTAA504.219526E-621.5932791
ATCATCG851.5825208E-1020.6406333
GCCTAAG559.62503E-619.6302532
CTGAGTG903.4015102E-1019.493934
ACGCTGA903.4015102E-1019.493931
GGTCGCT350.005517828719.28477718
CTATACC350.00552621619.2797133
AACAGCA350.00552621619.2797135
AACTCAG853.5342964E-919.0528932
CAACTCA957.003109E-1018.4679341
Produced by FastQC (version 0.11.2)