FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_63.3520000005ea52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_63.3520000005ea52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62780
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC7911.2599553998088562No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4470.7120101943294043No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2250.35839439311882765No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2130.33928002548582353No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1780.28352978655622807No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1770.28193692258681113No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1650.26282255495380696No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1550.2468939152596368No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1510.24052245938196878No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1450.2309652755654667No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC1380.21981522777954765No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1220.19432940426887543No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1180.18795794839120739No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT1160.18477222045237338No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA1060.16884358075820324No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA1020.1624721248805352No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT770.12265052564510989No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA750.11946479770627588No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA750.11946479770627588No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT740.11787193373685888No Hit
GCTCAGCTATGCCAACGTACTCTGCGTTGATAC730.11627906976744186No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA710.11309334182860784No Hit
GTTCATAAATAAATAAAAATGTATACAATGTTT670.10672188595093979No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA630.10035043007327175No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG257.761198E-426.9904385
TGAGTGA200.00856434926.99043810
GATACCA1500.024.33016626
ATACCAC1550.023.54532427
CCTATAC352.0141399E-423.134662
ACCTATA352.0141399E-423.134661
TGATACC1700.021.46779425
TTGGGAA450.001097010717.99362419
TTTGGGA450.001097010717.99362418
ACAGAAA500.00219756516.22011222
CTATACC500.002221154316.1942633
GTTCAGC500.002221154316.1942637
GAATGCA550.004147493314.74555623
GTTTGGG550.004191799614.72205617
TTGATAC2650.013.77179224
TATCAAC3050.013.7164531
GCAGCCC600.00738597413.5167627
ATGCAGC600.00738597413.5167625
AATGCAG600.00738597413.5167624
TGCAGCC600.00738597413.5167626
Produced by FastQC (version 0.11.2)