FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_55.3520000005e953.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_55.3520000005e953.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49341
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGCTGAAGACGTACTCTGCGTTGATACCA4860.9849820635982246No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4470.9059402930625645No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA4260.8633793396972091No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA4180.8471656431770738No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA3830.7762307209014816No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA3240.6566547090654831No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA2840.5755862264648062No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA2380.4823574714740277No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG2310.4681704870189092No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA2150.4357430939786385No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA1830.3708883078980969No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA1560.31616708214264No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC1490.30198009768752154No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA1490.30198009768752154No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1370.2776595529073185No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA1290.2614458563871831No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA1240.2513122960620985No Hit
AACGCAGAGTGAATGGGCATTGCTGAAAAAAAA1230.24928558399708156No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA1200.24320544780203077No Hit
GGTATCAACGCAGAGTGAATGGGCATTGCTGAA1200.24320544780203077No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1160.23509859954196308No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA1110.22496503921687847No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA1090.22091161508684462No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA1090.22091161508684462No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA1060.21483147889179385No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA980.19861778237165845No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAA950.19253764617660768No Hit
CCGCATTGCTGAAGACGTACTCTGCGTTGATAC910.18443079791654No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA900.18240408585152307No Hit
GTCTTCAGCAAAAAAAAAAAAAAAAAAAAAAAA870.1763239496564723No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC830.1682171013964046No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAA790.16011025313633692No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT780.15808354107132No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA770.15605682900630308No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA750.15200340487626923No Hit
GCTGAAGACGTACTAAAAAAAAAAAAAAAAAAA740.1499766928112523No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA740.1499766928112523No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA730.1479499807462354No Hit
GCTGAAGACGTAAAAAAAAAAAAAAAAAAAAAA730.1479499807462354No Hit
GAGTACGTCTTCAGCAAAAAAAAAAAAAAAAAA700.14186984455118462No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA700.14186984455118462No Hit
GCATTGCTGAAGACGTACAAAAAAAAAAAAAAA680.13781642042115075No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA680.13781642042115075No Hit
GTGAATGGGGCATTGCTGAAAAAAAAAAAAAAA670.13578970835613385No Hit
GTGAATGGGGCATTGCTGAAGAAAAAAAAAAAA670.13578970835613385No Hit
GAGTGAATGGGCATTGCTGAAGACAAAAAAAAA630.12768286009606614No Hit
TTGCTGAAGAAAAAAAAAAAAAAAAAAAAAAAA580.11754929977098154No Hit
TATCAACGCAGAGTGAATGGGGCATTGCTGAAG580.11754929977098154No Hit
GAATGGGCATTGCTGAAGAAAAAAAAAAAAAAA570.11552258770596462No Hit
GCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.11552258770596462No Hit
GCATTGCTGAAGACGTACTCAAAAAAAAAAAAA560.11349587564094768No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA520.10538902738087999No Hit
GCATTGCTGAAAAAAAAAAAAAAAAAAAAAAAA520.10538902738087999No Hit
TTGCTGAAGACAAAAAAAAAAAAAAAAAAAAAA520.10538902738087999No Hit
GTGAATGGGCATTGCTGAAGACGAAAAAAAAAA520.10538902738087999No Hit
GCGCATTGCTGAAGACGTACTCTGCGTTGATAC510.10336231531586307No Hit
CATTGCTGAAGACGTACTCTGCGTTGATACCAC510.10336231531586307No Hit
TTGCTGAAGACGTACTCAAAAAAAAAAAAAAAA510.10336231531586307No Hit
GTATCAACGCAGAGTGAATGGGGCATTGCTGAA500.10133560325084616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAC257.707508E-427.0081371
ATCTACG257.7535457E-426.980692
TCTACGC257.7535457E-426.980693
GATACCA751.6221748E-819.84634827
TGATACC751.6221748E-819.84634826
CGCTGAG350.00548210819.2719212
CTGAGTG350.00548210819.2719214
GCTGAGT350.00548210819.2719213
TGAGTGA350.00548210819.2719215
TCGCATT703.828718E-617.344737
ATGGGAG751.3466305E-414.3897025
TGAAAGG751.3466305E-414.3897022
TCTGCGT8900.013.68364127
GTGAAAG802.3092254E-413.5040691
ACTGCAT600.00745211713.4903466
CTCAAAA1304.208232E-813.49034413
TATCAAC4550.013.0588791
CGGCATT956.87371E-512.7803287
CAGCATT1101.9666175E-512.2639517
GTACTCA1451.7529783E-712.09479310
Produced by FastQC (version 0.11.2)