FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_48.3520000005e87d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_48.3520000005e87d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66861
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA16732.502206069307967No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA3140.4696310255604912No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA3060.4576659038901602No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA2940.4397182213846637No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2600.388866454285757No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA2460.3679274913626778No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA2310.3454928882308072No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2020.3021193221758574No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA2000.2991280417582746No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1980.2961367613406919No Hit
GAGTGAATGGGTTCCACAAAAAAAAAAAAAAAA1940.29015420050552637No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC1890.2826759994615695No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1740.2602413963296989No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1740.2602413963296989No Hit
TATCAACGCAGAGTGAATGGGTTCCACAAAAAA1710.2557544757033248No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA1590.23780679319782833No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1580.23631115298903696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.23182423236266284No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1530.2288329519450801No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1410.2108852694395836No Hit
ACGCAGAGTGAATGGGTTCCACGCAAAAAAAAA1210.18097246526375615No Hit
GTGAATGGGTTCCAAAAAAAAAAAAAAAAAAAA1170.17498990442859066No Hit
ACGCAGAGTGAATGGGGTTCCACAAAAAAAAAA1170.17498990442859066No Hit
GAGTGAATGGGGTTCCACAAAAAAAAAAAAAAA1140.17050298380221654No Hit
GAGTGAATGGGTTCCACGCAAAAAAAAAAAAAA1110.16601606317584242No Hit
GTGAATGGGGTTCCACGCAAAAAAAAAAAAAAA1100.16452042296705105No Hit
TATCAACGCAGAGTGAATGGGGTTCCACAAAAA1030.15405094150551144No Hit
AACGCAGAGTGAATGGGTTCCACAAAAAAAAAA980.14657274046155458No Hit
GTTCCACAAAAAAAAAAAAAAAAAAAAAAAAAA980.14657274046155458No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCAAAA970.14507710025276319No Hit
GTGAATGGGGTTCCACGAAAAAAAAAAAAAAAA960.14358146004397182No Hit
GTGAATGGGTTCCACGAAAAAAAAAAAAAAAAA930.1390945394175977No Hit
GTTCCACGCTTAACGTACTCTTCGTTGATACCA920.13759889920880633No Hit
CGTTAAGCGTGGAAAAAAAAAAAAAAAAAAAAA880.13161633837364084No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA870.13012069816484945No Hit
GTGAATGGGGGTTCCACAAAAAAAAAAAAAAAA830.12413813732968398No Hit
GAGTGAATGGGTTCCAAAAAAAAAAAAAAAAAA830.12413813732968398No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA820.1226424971208926No Hit
GTGAATGGGAGTTCCACAAAAAAAAAAAAAAAA810.12114685691210123No Hit
AACGCAGAGTGAATGGGTTCCACGCAAAAAAAA810.12114685691210123No Hit
ACGCAGAGTGAATGGGTTCCAAAAAAAAAAAAA780.1166599362857271No Hit
GTACGTTAAGCGTGGAAAAAAAAAAAAAAAAAA770.11516429607693573No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA700.10469481461539612No Hit
GTATCAACGCAGAGTGAATGGGTTCCACAAAAA690.10319917440660474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTG257.7454146E-427.0029955
CAGACGT257.7454146E-427.0029954
CGTGTGC257.7454146E-427.0029958
GTGTGCT257.7454146E-427.0029959
ACGTGTG257.7454146E-427.0029957
GTGCTCT200.00858013126.98279811
TGTGCTC257.7794085E-426.98279610
GACGTGT300.002241078822.5024956
GATACCA2250.021.60239627
TGATACC2400.020.81480826
TTGATAC2750.018.16565125
GAATGAG450.001094599718.0019975
CGTTGAT3050.016.37886623
GTTGATA3000.016.20179724
TGGGGAT550.00418262514.7289076
TGAATGA550.00418262514.7289074
TGGGGAG700.0013155613.5014966
AGGTTCC700.0013155613.5014967
TATCAAC6400.013.4913991
TACTCAA853.919765E-412.69778710
Produced by FastQC (version 0.11.2)