FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_47.3520000005e854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_47.3520000005e854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107137
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA5750.5366960060483307No Hit
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA4490.4190895768968704No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA4450.4153560394634907No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC4040.37708728077134884No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA3780.35281928745438085No Hit
GTCCAGTCTGCTAACGTACTCTGCGTTGATACC3160.2949494572369956No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT3070.2865489980118913No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA3040.28374884493685654No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA3000.28001530750347686No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA2950.27534838571175224No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA2880.2688146952033378No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.266014542128303No Hit
CTCCAGTCTGCTAACGTACTCTGCGTTGATACC2760.2576140829031987No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA2340.21841193985271193No Hit
CCAGTCTGCTAACGTACTCTGCGTTGATACCAC2200.205344558835883No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.19881086832746858No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA1780.16614241578539626No Hit
GTGAATGGGTCCAGTCAAAAAAAAAAAAAAAAA1690.15774195656029197No Hit
GCTCCAGTCTGCTAACGTACTCTGCGTTGATAC1690.15774195656029197No Hit
GTGAATGGGGAGTACGTTAGCAAAAAAAAAAAA1650.15400841912691227No Hit
GTACGTTAGCAAAAAAAAAAAAAAAAAAAAAAA1640.15307503476856735No Hit
CTTCCAGTCTGCTAACGTACTCTGCGTTGATAC1620.1512082660518775No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA1590.14840811297684273No Hit
ACTCCAGTCTGCTAACGTACTCTGCGTTGATAC1550.14467457554346302No Hit
GTTCCAGTCTGCTAACGTACTCTGCGTTGATAC1510.14094103811008335No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.13720750067670365No Hit
ATCCAGTCTGCTAACGTACTCTGCGTTGATACC1440.1344073476016689No Hit
CATCCAGTCTGCTAACGTACTCTGCGTTGATAC1380.12880704145159935No Hit
TCTCCAGTCTGCTAACGTACTCTGCGTTGATAC1360.1269402727349095No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA1270.11853981350980519No Hit
ATTCCAGTCTGCTAACGTACTCTGCGTTGATAC1220.11387289171808058No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.11200612300139073No Hit
CGTTAGCAGACTGGAAAAAAAAAAAAAAAAAAA1200.11200612300139073No Hit
GAGTGAATGGGATCCAGTCAAAAAAAAAAAAAA1150.10733920120966611No Hit
GTACGTTAGCAGACTGAAAAAAAAAAAAAAAAA1130.10547243249297628No Hit
GTGAATGGGTCCAGTCTGAAAAAAAAAAAAAAA1100.1026722794179415No Hit
GTGAATGGGTCCAGTCTAAAAAAAAAAAAAAAA1080.10080551070125168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGA200.00858698926.9929921
GGGGAAG200.00858698926.9929927
TATCTAC300.00225471422.4941581
TGATACC1700.020.65134827
CCTTATC404.995366E-420.2447432
AGTTCCA404.995366E-420.2447436
TGGGGAA350.005509077619.2807086
CTTATCC500.002233585816.1957933
TAAGCAT801.3860361E-515.1835584
AAGCATA801.3860361E-515.1835585
TTGATAC4200.014.78878727
CCACTCC550.004214973714.723455
GTTGATA4250.014.614826
GTTTAAG751.3650417E-414.3962611
TTTAAGC751.3650417E-414.3962612
TTAAGCA852.4579444E-514.2904063
ATTTCCA802.3608012E-413.4964966
CATATCA700.001328372313.4964958
GCATATC853.9416694E-412.7025837
GTCTCCA750.002232865212.5967296
Produced by FastQC (version 0.11.2)