FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_32.3520000005e68c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_32.3520000005e68c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113735
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA22892.0125730865608653No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA17601.5474568074910977No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAC17511.5395436760891545No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA11941.0498087659911197No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA10430.917044005802963No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA9990.8783575856156856No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA9130.802743218886007No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA9060.79658856112894No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA7900.6945970897261177No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA6960.6119488284169341No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA6800.5978810392579241No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA6230.5477645403789511No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT5790.5090781201916736No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC5610.49325185738778743No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA5460.4800633050512155No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA5110.4492900162658812No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA4970.4369807007517475No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA4930.43346375346199495No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA4870.4281883325273662No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA4810.42291291159273753No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA4780.42027520112542316No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.39213962280740317No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC4390.3859849650503363No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA4370.38422649140546006No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA4160.3657625181342594No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3960.348177781685497No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA3640.3200422033674771No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA3600.3165252560777245No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA3530.31037059832065766No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG3440.30245746691871456No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA3410.2998197564514002No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA3380.2971820459840858No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA3330.2927858618718952No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA3320.291906625049457No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC3210.28223502000263767No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC2990.26289180990899896No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA2960.2602540994416846No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA2810.24706554710511278No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA2750.241790126170484No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA2700.23739394205829342No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC2680.23563546841341715No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA2550.22420538972172155No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA2500.21980920560953093No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA2460.2162922583197784No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA2410.21189607420758783No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA2340.20574141645052094No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA2310.2031037059832066No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA2240.1969490482261397No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA2240.1969490482261397No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA2170.19079439046907284No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA2060.1811227854222535No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA2050.18024354859981537No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA2050.18024354859981537No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA2040.17936431177737724No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA1990.17496812766518663No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA1960.17233041719787223No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA1930.1696927067305579No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.16881346990811974No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA1830.16090033850617663No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.15826262803886226No Hit
ATCAACGCAGAGTGAATGGGGCTAACTATCCAC1780.15650415439398602No Hit
GAGTGAATGGGCTAACTAAAAAAAAAAAAAAAA1770.1556249175715479No Hit
GTGAATGGGCTAACTATCAAAAAAAAAAAAAAA1750.15386644392667165No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC1740.15298720710423352No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC1710.15034949663691916No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA1670.14683254934716666No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.14155712841253792No Hit
CTAACTATCCACGTACAAAAAAAAAAAAAAAAA1600.14067789159009977No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA1570.13804018112278543No Hit
AACTATCCACGTACAAAAAAAAAAAAAAAAAAA1570.13804018112278543No Hit
CTATCCACGTACTAAAAAAAAAAAAAAAAAAAA1550.13628170747790916No Hit
ACGCAGAGTGAATGGGGCTAACTAAAAAAAAAA1550.13628170747790916No Hit
GTGAATGGGGCTAACTATAAAAAAAAAAAAAAA1540.13540247065547104No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA1530.13452323383303294No Hit
CTAACTATCCACAAAAAAAAAAAAAAAAAAAAA1500.13188552336571854No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA1500.13188552336571854No Hit
GCTAACTATCCACGTACTCAAAAAAAAAAAAAA1470.1292478128984042No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1470.1292478128984042No Hit
GTGAATGGGGCTAACAAAAAAAAAAAAAAAAAA1450.12748933925352793No Hit
GGGCTAACTATCCACGTACTCAAAAAAAAAAAA1450.12748933925352793No Hit
CGGCTAACTATCCACGTACTCTGCGTTGATACC1440.1266101024310898No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA1420.12485162878621357No Hit
GAGTGAATGGGCTAACTATAAAAAAAAAAAAAA1410.12397239196377545No Hit
TATCAACGCAGAGTGAATGGGCTAACTATAAAA1380.12133468149646107No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA1370.12045544467402294No Hit
AACTATCCACGTACTCAAAAAAAAAAAAAAAAA1350.11869697102914671No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA1310.1151800237393942No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.11166307644964171No Hit
CTAACTATCCACGTACTCTGCGTTGATACCACT1240.10902536598232734No Hit
CCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.10374994504769859No Hit
GAATGGGGCTAACTATCCACGTACTCAAAAAAA1180.10374994504769859No Hit
CTATCCACGTACCAAAAAAAAAAAAAAAAAAAA1170.10287070822526047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA200.00860906726.9801865
GTATCAT653.6379788E-1226.9801851
TATCATC701.0913936E-1125.053032
TCGCAGA703.110472E-1023.1258747
ATCGCAG703.110472E-1023.1258746
ATCATCG757.385097E-1021.584153
TCATCGC757.385097E-1021.584154
CTACGCA1000.021.5841464
TCTACGC951.8189894E-1221.3001463
ACGCTGA707.867129E-921.1987171
TACGCAG1050.020.5563325
CATCGCA801.6516424E-920.235145
TATCTAC1003.6379788E-1220.2351381
CGCTGAG751.7402272E-819.7854712
CTGAGTG751.7402272E-819.7854714
GCTGAGT751.7402272E-819.7854713
TGAGTGA751.7402272E-819.7854715
ATCTACG1057.2759576E-1219.271562
TGGGAGG350.0055262519.271566
AACGTAG602.0331752E-517.986791
Produced by FastQC (version 0.11.2)