FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_16.3520000005e48e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_16.3520000005e48e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105094
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCTATGGTGAAAAAAAAAAAAAAAA14331.3635412107256362No Hit
GCTATGGTGTTCACGTACTCTGCGTTGATACCA13551.2893219403581557No Hit
GTGAATGGGCTATGGTGTAAAAAAAAAAAAAAA8900.8468609054750985No Hit
ACGCAGAGTGAATGGGCTATGGTGAAAAAAAAA6140.5842388718670904No Hit
GTGAATGGGGCTATGGTGAAAAAAAAAAAAAAA5930.5642567606143072No Hit
CCGCTATGGTGTTCACGTACTCTGCGTTGATAC5770.549032294897901No Hit
GTGTTCACGTACTCAAAAAAAAAAAAAAAAAAA5390.5128741888214361No Hit
GGGCTATGGTGAAAAAAAAAAAAAAAAAAAAAA5230.49764972310502975No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGAAAA4770.4538793841703618No Hit
GAGTGAATGGGCTATGGTGAAAAAAAAAAAAAA4170.3967876377338383No Hit
GTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.38727234666108434No Hit
ACGCAGAGTGAATGGGCTATGGTGTAAAAAAAA3900.3710963518374027No Hit
GCTATGGTGTAAAAAAAAAAAAAAAAAAAAAAA3750.3568234152282719No Hit
AACGCAGAGTGAATGGGCTATGGTGAAAAAAAA3550.33779283308276403No Hit
GGGCTATGGTGTAAAAAAAAAAAAAAAAAAAAA3530.33588977486821325No Hit
GTGAATGGGCTATGGTAAAAAAAAAAAAAAAAA3510.3339867166536624No Hit
GCTATGGTGAAAAAAAAAAAAAAAAAAAAAAAA3460.3292290711172855No Hit
GCGCTATGGTGTTCACGTACTCTGCGTTGATAC3380.3216168382590823No Hit
CTATGGTGTTCACGTACTCTGCGTTGATACCAC3290.31305307629360385No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTAAA3080.2930709650408206No Hit
CTGCTATGGTGTTCACGTACTCTGCGTTGATAC2950.28070108664624055No Hit
GTGAATGGGCTATGGAAAAAAAAAAAAAAAAAA2840.27023426646621124No Hit
GGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.2692827373589358No Hit
ACGCTATGGTGTTCACGTACTCTGCGTTGATAC2690.2559613298570803No Hit
GAGTGAATGGGGCTATGGTGAAAAAAAAAAAAA2630.250252155213428No Hit
GGGCTATGGTGTTCACGTACTCTGCGTTGATAC2560.24359145146250022No Hit
GAATGGGCTATGGTGAAAAAAAAAAAAAAAAAA2560.24359145146250022No Hit
GTGCTATGGTGTTCACGTACTCTGCGTTGATAC2550.24263992235522483No Hit
GGCTATGGTGAAAAAAAAAAAAAAAAAAAAAAA2550.24263992235522483No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGAAA2480.23597921860429708No Hit
GAGTGAATGGGCTATGGTGTAAAAAAAAAAAAA2330.22170628199516623No Hit
CAGCTATGGTGTTCACGTACTCTGCGTTGATAC2310.21980322378061545No Hit
CGGCTATGGTGTTCACGTACTCTGCGTTGATAC2090.19886958342055683No Hit
ATGCTATGGTGTTCACGTACTCTGCGTTGATAC2070.19696652520600605No Hit
ATCAACGCAGAGTGAATGGGCTATGGTGAAAAA1850.17603288484594742No Hit
AACGCAGAGTGAATGGGCTATGGTGTAAAAAAA1820.17317829752412128No Hit
GAATGGGGCTATGGTGAAAAAAAAAAAAAAAAA1820.17317829752412128No Hit
TCGCTATGGTGTTCACGTACTCTGCGTTGATAC1820.17317829752412128No Hit
GAGCTATGGTGTTCACGTACTCTGCGTTGATAC1760.16746912288046892No Hit
GTGAATGGGGCTATGGTGTAAAAAAAAAAAAAA1720.16366300645136736No Hit
GTGAATGGGGCTATGGAAAAAAAAAAAAAAAAA1720.16366300645136736No Hit
GCTATGGTGTTCACGTACTCAAAAAAAAAAAAA1680.15985689002226577No Hit
GTGAATGGGGCTATGGTAAAAAAAAAAAAAAAA1610.15319618627133805No Hit
ACGCAGAGTGAATGGGCTATGGTAAAAAAAAAA1570.14939006984223646No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1560.14843854073496107No Hit
GGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.14368089519858412No Hit
GGCTATGGTGTTCACGTACTCTGCGTTGATACC1490.14177783698403335No Hit
ACGCAGAGTGAATGGGGCTATGGTGAAAAAAAA1470.13987477876948254No Hit
GAATGGGCTATGGTGTAAAAAAAAAAAAAAAAA1440.1370201914476564No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1440.1370201914476564No Hit
CTATGGTGTAAAAAAAAAAAAAAAAAAAAAAAA1440.1370201914476564No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGTAA1390.13226254591127942No Hit
GGCTATGGTAAAAAAAAAAAAAAAAAAAAAAAA1370.13035948769672864No Hit
TATCAACGCAGAGTGAATGGGGCTATGGTGAAA1320.1256018421603517No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1300.12369878394580092No Hit
TTGCTATGGTGTTCACGTACTCTGCGTTGATAC1300.12369878394580092No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.1227472548385255No Hit
AAGCTATGGTGTTCACGTACTCTGCGTTGATAC1290.1227472548385255No Hit
GTTCACGTACTCAAAAAAAAAAAAAAAAAAAAA1260.11989266751669934No Hit
ACGCAGAGTGAATGGGCTATGGAAAAAAAAAAA1230.11703808019487315No Hit
GGCTATGGTGTAAAAAAAAAAAAAAAAAAAAAA1220.11608655108759777No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1220.11608655108759777No Hit
CAACGCAGAGTGAATGGGCTATGGTGAAAAAAA1200.114183492873047No Hit
CGCTATGGTGTTCACGTACTCTGCGTTGATACC1180.11228043465849619No Hit
TAGCTATGGTGTTCACGTACTCTGCGTTGATAC1140.10847431822939464No Hit
GGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.10752278912211925No Hit
GTGAATGGGCTATGGTGTTCACGTACTCAAAAA1130.10752278912211925No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1130.10752278912211925No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1120.10657126001484385No Hit
GGGCTATGGTGTTCACGTACTCAAAAAAAAAAA1120.10657126001484385No Hit
TATCAACGCAGAGTGAATGGGCTATGGTAAAAA1060.1008620853711915No Hit
ATCAACGCAGAGTGAATGGGCTATGGTGTAAAA1060.1008620853711915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG307.092472E-527.0028612
GTATCAT505.127731E-927.0028611
TATCTAC307.092472E-527.0028611
TCTACGC257.7970664E-426.989993
TACGCAG257.7970664E-426.989995
CTACGCA257.7970664E-426.989994
CAACTCA703.074092E-1023.1453111
GTATGGG300.002255877222.4916573
CTGAGTG603.5792254E-822.4916574
ACTCAGA757.3305273E-1021.5919933
ACGCTGA658.305142E-820.7714311
TATCATC658.305142E-820.7714312
AACTCAG801.6298145E-920.2521462
TCAGAGT801.6389095E-920.2424935
CTCAGAG801.6389095E-920.2424934
AACGTAG559.523135E-619.6384451
ACGTAGA559.523135E-619.6384452
TGAGTGA701.8236642E-719.2785665
CGTAGAG501.02630256E-418.8929943
GTAGAGT450.001103850917.9933264
Produced by FastQC (version 0.11.2)