FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_15.3520000005e464.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_15.3520000005e464.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108215
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC14101.3029616966224646No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA7170.6625698840271681No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA6790.6274546042600379No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA5690.5258051101972925No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA4370.4038257173219979No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA4330.4001293720833526No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT4270.3945848542253847No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA4160.38441990481911015No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA4160.38441990481911015No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA3980.3677863512452063No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA3830.35392505660028645No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA3510.32435429469112415No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT3230.2984798780206071No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3180.2938594464723005No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA2940.2716813750404288No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA2810.2596682530148316No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC2740.2531996488472023No Hit
ACTCTAACTATGCCAACGTACTCAAAAAAAAAA2710.25042738991821833No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA2260.20884350598345888No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA2060.19036177979023242No Hit
GCTATGCCAACGTACTCAAAAAAAAAAAAAAAA2000.18481726193226447No Hit
ATGCCAACGTACTCAAAAAAAAAAAAAAAAAAA1940.17927274407429653No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA1720.15894284526174746No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.1570946726424248No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA1660.1533983274037795No Hit
GAGTACGTTGGCAAAAAAAAAAAAAAAAAAAAA1600.14785380954581157No Hit
GCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.14323337799750496No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT1450.13399251490089176No Hit
GCTATGCCAAAAAAAAAAAAAAAAAAAAAAAAA1410.13029616966224647No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA1380.1275239107332625No Hit
GTGAATGGGGCGACCCCAGGTCAGGCGGGATTA1380.1275239107332625No Hit
GTGAATGGGTGTGAGCAACTCTAACTATGCCAA1360.12567573811393984No Hit
GCCCTCAGCTATGCCAACGTACTCTGCGTTGAT1340.1238275654946172No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC1310.12105530656563322No Hit
GCTCAGCTATGCCAACGTACTCTGCGTTGATAC1260.1164348750173266No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT1260.1164348750173266No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA1180.10904218454003604No Hit
CAGCTATGCCAAAAAAAAAAAAAAAAAAAAAAA1170.10811809823037472No Hit
GCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.10811809823037472No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT1170.10811809823037472No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.10626992561105207No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.10626992561105207No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA1140.10534583930139074No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTT1140.10534583930139074No Hit
GTGAATGGGTCAGCTAAAAAAAAAAAAAAAAAA1110.10257358037240678No Hit
GGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT1090.10072540775308413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG200.00860737826.9800951
TATCTAC200.00860737826.9800951
ATACCAC2000.023.6513827
CAACTCA401.8440625E-523.6075821
GTATCAT300.002261120422.4834121
GATACCA2200.021.50125326
TGATACC2400.019.70948425
TTCTCAG350.00550986219.280426
TATCATC350.00552464419.2714942
TGAAAGG1006.730261E-1118.8860652
GTGAAAG1300.017.6408311
TTGATAC3250.014.97054424
CCTCAGA1004.6067362E-714.84592412
CGCTCAA550.00421571314.7232311
GACGTCA550.00421571314.7232310
AATGGAA550.00421571314.723234
TATCAAC8700.013.955221
ATGGGAG2000.013.4962955
TGGGGTG600.00750656913.4962956
TGGGAGA802.3614036E-413.4962956
Produced by FastQC (version 0.11.2)