FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate5_1.3520000005e29d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate5_1.3520000005e29d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA171.6666666666666667No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA151.4705882352941175No Hit
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA151.4705882352941175No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA111.0784313725490196No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA90.8823529411764706No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.8823529411764706No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA90.8823529411764706No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA90.8823529411764706No Hit
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA80.7843137254901961No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA70.6862745098039216No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.6862745098039216No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.6862745098039216No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA60.5882352941176471No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA60.5882352941176471No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA50.49019607843137253No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA50.49019607843137253No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.49019607843137253No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA50.49019607843137253No Hit
CAACTCAGAGTGAATGGGCAGACACAAAAAAAA50.49019607843137253No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA50.49019607843137253No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.49019607843137253No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA40.39215686274509803No Hit
CACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
GAATGGGCAGACACAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
AACGCAGAGTGAATGGGCAGACACACGAAAAAA40.39215686274509803No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT30.29411764705882354No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA30.29411764705882354No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACAAA30.29411764705882354No Hit
CAACGCAGAGTGAATGGGCAGACAAAAAAAAAA30.29411764705882354No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA30.29411764705882354No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC30.29411764705882354No Hit
GTATCATCGCAGAGTGAATGGGCAGACACAAAA30.29411764705882354No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA30.29411764705882354No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC30.29411764705882354No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA30.29411764705882354No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGT30.29411764705882354No Hit
TATCTACGCAGAGTGAATGGGCAGACAAAAAAA20.19607843137254902No Hit
GGTATCAACGCAGAGTGAATGGGCAGACAAAAA20.19607843137254902No Hit
ACGCAGAATGAATGGGCAGACACAAAAAAAAAA20.19607843137254902No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTATTAACGCAGAGTGAATGGGCAGACACAAAA20.19607843137254902No Hit
AACGCAGAGTGAATGGGCAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA20.19607843137254902No Hit
CAGACACACGTACTCAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACGCAAGAGTGAATGGGATGAGCAGACAAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA20.19607843137254902No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA20.19607843137254902No Hit
GAATGGGCAGACAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CGTCAGACATCGCAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CACACGTAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CCGCAAATGAGCAGACACACGTACTCTGCGTTG20.19607843137254902No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTATCATCGCAGAGTGAATGGGCAGACAAAAAA20.19607843137254902No Hit
GAGTGAATGGGGGCCTCAGAAAAAAAAAAAAAA20.19607843137254902No Hit
GAATGGGCCTCGTTCCACAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGCGTATCAGCTAAAAAAAAAAAAAA20.19607843137254902No Hit
GCTCATTATAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA20.19607843137254902No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTT20.19607843137254902No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGAAA20.19607843137254902No Hit
AACGTAGAGTGAATGGGCAGACACAAAAAAAAA20.19607843137254902No Hit
CACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAGTGTATGGGCAGACACACGTACTCAAAAAAA20.19607843137254902No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)