FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_94.3520000005e24d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_94.3520000005e24d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19146
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC1510.7886764859500678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.47529510080434556No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA660.34471952366029457No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC640.3342734774887705No Hit
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA470.24548208503081584No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA400.20892092343048155No Hit
ACTCCTTGCAGTAACGTACTCTGCGTTGATACC260.13579860022981302No Hit
GGGTACGTTACTGCAAAAAAAAAAAAAAAAAAA250.130575577144051No Hit
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.130575577144051No Hit
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA240.12535255405828893No Hit
GCTCCTTGCAGTAACGTACTCTGCGTTGATACC230.12012953097252689No Hit
GTGAATGGGGAGTACGTTACTGCAAAAAAAAAA210.10968348480100282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT5200.017.15298727
TATCAAC1153.9108272E-1016.4524021
CGCTCCT707.152025E-515.4045747
ATCAACG1201.2580131E-814.640682
TGCGTTG953.932084E-614.22576127
TCAACGC1151.2640521E-714.0650463
CTCTGCG6700.013.71618226
GCCTCCT903.913126E-513.4790037
CAACGCA1253.4969162E-712.9398424
CTGCGTT1901.6370905E-1112.80318527
GCTCCTT1654.800313E-912.2536388
ACTCTGC7600.011.91407525
CCTCCTT3350.011.668398
TACTCTG8000.011.31837224
GTAACGT8250.011.30301518
CAGTAAC8300.011.23492316
AGTAACG8300.011.23492317
AACGTAC8200.011.20712420
TAACGTA8200.011.20712419
ACGTACT8100.011.178637521
Produced by FastQC (version 0.11.2)