FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_92.3520000005e209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_92.3520000005e209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20649
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA1050.5084992009298271No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC880.42617075887452177No Hit
ACGCAGAGTGAATGGGCTAAACGTACTCAAAAA800.3874279626132016No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.3341566177538864No Hit
GTGAATGGGCTAAACGTACTCAAAAAAAAAAAA580.28088527289457116No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.21308537943726089No Hit
GTATCAACGCAGAGTGAATGGGCTAAACGTACT420.20339968037193082No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA410.1985568308392658No Hit
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA410.1985568308392658No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC410.1985568308392658No Hit
GCTAAACGTACTCAAAAAAAAAAAAAAAAAAAA360.17434258317594073No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACCT270.13075693738195554No Hit
AACGCAGAGTGAATGGGCAGAGTGGCAAAAAAA270.13075693738195554No Hit
GAGTGAATGGGCTAAACGTACTCAAAAAAAAAA270.13075693738195554No Hit
AACGCAGAGTGAATGGGCTAAACGTACTCAAAA270.13075693738195554No Hit
GTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.11622838878396048No Hit
AACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA230.11138553925129546No Hit
CCCCCAGAGTGGCTAAACGTACTCTGCGTTGAT230.11138553925129546No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT230.11138553925129546No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.11138553925129546No Hit
GAATGGGCAGAGTGGCAAAAAAAAAAAAAAAAA220.10654268971863044No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA210.10169984018596541No Hit
GAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA210.10169984018596541No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT257.5672165E-426.9999981
TATCATC257.5672165E-426.9999982
ATCATCG300.002190190122.53
GTTGATA404.7208936E-420.29951127
TCATCGC350.005353392619.2857134
TATCAAC1400.018.3214261
ATCAACG1600.015.18752
TCTGCGT4650.015.13368627
TCAACGC1703.6379788E-1113.53
GTATCAA700.001262892613.4999991
CAGAGAA956.456182E-512.78947414
CGTTGAT853.6208436E-412.73694827
CAACGCA1801.3096724E-912.0000014
GAGAAAA1152.8778815E-511.7391316
AGAGAAA1152.8778815E-511.7391315
CTCTGCG6350.011.08214826
AGAAAAA1401.778951E-510.607141517
TGGCTAA7350.010.10204114
ACTCTGC7000.010.05309125
AGTGGCT7300.09.986312
Produced by FastQC (version 0.11.2)