FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_91.3520000005e1d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_91.3520000005e1d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6723
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGCTAGACGTACTCTGCGTTGATACCAC1291.9187862561356537No Hit
ACATCCAGCTAGACGTACTCTGCGTTGATACCA470.699092666964153No Hit
GTGAATGGGACATCCAGCAAAAAAAAAAAAAAA420.6247211066488175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.5057266101442809No Hit
GTGAATGGGACATCCAAAAAAAAAAAAAAAAAA210.31236055332440876No Hit
GTGAATGGGACATCCAGAAAAAAAAAAAAAAAA210.31236055332440876No Hit
ATCCAGCTAGACGTACTCTGCGTTGATACCACT180.2677376171352075No Hit
GTGAATGGGACATCCAGCTAGAAAAAAAAAAAA170.25286330507214044No Hit
ACGCAGAGTGAATGGGACATCCAGCAAAAAAAA170.25286330507214044No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA150.22311468094600626No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCAAA150.22311468094600626No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA140.20824036888293915No Hit
CCCACATCCAGCTAGACGTACTCTGCGTTGATA130.19336605681987207No Hit
GTATCAACGCAGAGTGAATGGGACATCCAGCAA130.19336605681987207No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT120.178491744756805No Hit
CCCCACATCCAGCTAGACGTACTCTGCGTTGAT120.178491744756805No Hit
GTGAATGGGGACATCCAAAAAAAAAAAAAAAAA110.1636174326937379No Hit
AACGCAGAGTGAATGGGACATCCAGCAAAAAAA110.1636174326937379No Hit
CCCCCACATCCAGCTAGACGTACTCTGCGTTGA110.1636174326937379No Hit
ACGCAGAGTGAATGGGACATCCAAAAAAAAAAA110.1636174326937379No Hit
GAGTGAATGGGACATCCAGCAAAAAAAAAAAAA110.1636174326937379No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA100.14874312063067083No Hit
GTGAATGGGGACATCCAGAAAAAAAAAAAAAAA100.14874312063067083No Hit
GAATGGGACATCCAGCAAAAAAAAAAAAAAAAA100.14874312063067083No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA90.13386880856760375No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA90.13386880856760375No Hit
GCCACATCCAGCTAGACGTACTCTGCGTTGATA90.13386880856760375No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA90.13386880856760375No Hit
GTATCAACGCAGAGTGAATGGGACATCCAGCTA80.11899449650453667No Hit
ATCAACGCAGAGTGAATGGGACATCCAGCAAAA80.11899449650453667No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT70.10412018444146957No Hit
ACGCAGAGTGAATGGGACATCCAGCTAGAAAAA70.10412018444146957No Hit
GTATCAACGCAGAGTGAATGGGACATCCAAAAA70.10412018444146957No Hit
CCTACATCCAGCTAGACGTACTCTGCGTTGATA70.10412018444146957No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCTAG70.10412018444146957No Hit
ACGCAGAGTGAATGGGGACATCCAAAAAAAAAA70.10412018444146957No Hit
TATCAACGCAGAGTGAATGGGACATCCAGAAAA70.10412018444146957No Hit
GAGTGAATGGGGACATCCAAAAAAAAAAAAAAA70.10412018444146957No Hit
ACGCAGAGTGAATGGGACATCCAGAAAAAAAAA70.10412018444146957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC653.1854823E-516.6153851
AATGGGG550.003685335214.7272734
TCAACGC751.085242E-414.43
ATCAACG751.085242E-414.42
TCTGCGT1551.6927515E-812.19354827
CAACGCA905.1014917E-412.0000014
AACGCAG1050.001861110.2857145
CAGAGTG1200.0056397629.09
AGAGTGA1200.0056397629.010
ACGCAGA1250.0078948478.646
GCAGAGT1250.0078948478.648
CGCAGAG1250.0078948478.647
CTCTGCG2151.6542888E-58.1627926
CACATCC1500.00360573748.0999999
ACTCTGC2252.937733E-57.799999725
GTACTCT2303.8737984E-57.630434524
TACTCTG2355.0744144E-57.468085324
GAATGGG2006.6186895E-47.4253
TGAATGG2006.6186895E-47.4252
ATCCAGC3103.675632E-77.403225412
Produced by FastQC (version 0.11.2)