FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_90.3520000005e1b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_90.3520000005e1b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7742
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA330.42624644794626715No Hit
GTGAATGGGCAGTCTCAAAAAAAAAAAAAAAAA300.38749677086024287No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC210.27124773960216997No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA200.25833118057349524No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA200.25833118057349524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.24541462154482047No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.20666494445879618No Hit
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA160.20666494445879618No Hit
TATCAACGCAGAGTGAATGGGCAGTCTCTGGAA150.19374838543012143No Hit
ACGCAGAGTGAATGGGCAGTCTCTGGAAAAAAA140.18083182640144665No Hit
TATCAACGCAGAGTGAATGGGCAGTCTCAAAAA140.18083182640144665No Hit
GAGTACGTTTCCAAAAAAAAAAAAAAAAAAAAA140.18083182640144665No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1679152673727719No Hit
ACTCTGCGTTGATACCAAAAAAAAAAAAAAAAA130.1679152673727719No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG130.1679152673727719No Hit
GTATCAACGCAGAGTGAATGGGAACGTACTCTG130.1679152673727719No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.15499870834409715No Hit
ACGCAGAGTGAATGGGAACGTACTCTGCGTTGA110.14208214931542237No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA110.14208214931542237No Hit
AACGCAGAGTGAATGGGCAGTCTCAAAAAAAAA110.14208214931542237No Hit
AACGCAGAGTGAATGGGCAGTCTCTGGAAAAAA110.14208214931542237No Hit
AACGTACTCTGCGTTGAAAAAAAAAAAAAAAAA100.12916559028674762No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA100.12916559028674762No Hit
GTGAATGGGACAGTCTCAAAAAAAAAAAAAAAA100.12916559028674762No Hit
ATCAACGCAGAGTGAATGGGAACGTACTCTGCG100.12916559028674762No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA100.12916559028674762No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT100.12916559028674762No Hit
GTGAATGGGACAGTCTCTGGAAAAAAAAAAAAA100.12916559028674762No Hit
GTGAATGGGCAGTCTCTGGAAAAAAAAAAAAAA100.12916559028674762No Hit
GTGAATGGGGAGTACGTTTCCAAAAAAAAAAAA90.11624903125807284No Hit
ACGCAGAGTGAATGGGCAGTCTCAAAAAAAAAA90.11624903125807284No Hit
GAAACGTACTCTGCGAAAAAAAAAAAAAAAAAA90.11624903125807284No Hit
GAGTGAATGGGCAGTCTCAAAAAAAAAAAAAAA90.11624903125807284No Hit
GAACGTACTCTGCGTTGATACAAAAAAAAAAAA90.11624903125807284No Hit
GTACTCTGCGTTGATACAAAAAAAAAAAAAAAA90.11624903125807284No Hit
GTCTCTGGAAAAAAAAAAAAAAAAAAAAAAAAA90.11624903125807284No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA90.11624903125807284No Hit
ATCAACGCAGAGTGAATGGGCAGTCTCTGGAAA90.11624903125807284No Hit
GTGAATGGGAAACGTACTCTGCGTAAAAAAAAA90.11624903125807284No Hit
ACGCAGAGTGAATGGGGAGTACGTTTCCAAAAA90.11624903125807284No Hit
CCCCCAGTCTCTGGAAACGTACTCTGCGTTGAT90.11624903125807284No Hit
AACGTACTCTGCGTTAAAAAAAAAAAAAAAAAA80.10333247222939809No Hit
GAGTGAATGGGAACGTACTCTGCGTTGATACAA80.10333247222939809No Hit
GTGAATGGGGCAGTCAAAAAAAAAAAAAAAAAA80.10333247222939809No Hit
GTGAATGGGGCAGTCTCTGGAAAAAAAAAAAAA80.10333247222939809No Hit
GAGTGAATGGGGCAGTCTCAAAAAAAAAAAAAA80.10333247222939809No Hit
GTGAATGGGAACGTACTCAAAAAAAAAAAAAAA80.10333247222939809No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA80.10333247222939809No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC459.793778E-418.0000022
GTATCAA500.00198545216.21
TCAACGC600.00668888713.54
ATCAACG600.00668888713.53
TCTGCGT2150.012.5581427
GCAGTCT1203.6505957E-511.259
GGCAGTC900.007513899410.5000018
CCAGTCT1257.055138E-49.728
CTCTGCG2901.2187229E-109.31034426
TGGAAAC2704.14002E-99.016
CTCTGGA2901.3478711E-98.84482713
TCTCTGG2901.3478711E-98.84482712
GTCTCTG3002.4974725E-98.54999911
TCTGGAA2851.0537406E-88.52631614
CTGGAAA2851.0537406E-88.52631615
AGTCTCT3053.3687684E-98.40983610
GTGAATG2253.1938562E-68.4000011
TGAATGG2253.1938562E-68.4000012
GGAAACG2901.4215402E-88.3793117
GAAACGT3002.5438567E-88.09999918
Produced by FastQC (version 0.11.2)