FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_9.3520000005d7a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_9.3520000005d7a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50296
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC4690.932479720057261No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC2210.4393987593446795No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA2110.4195164625417528No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA1960.38969301733736283No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1700.33799904564975347No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA1520.30221091140448547No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC1510.3002226817241928No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC1340.2664227771592174No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1290.2564816287577541No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1290.2564816287577541No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA1230.24455225067599812No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1150.22864641323365678No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA1090.21671703515190074No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC980.19484650866868142No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA950.1888818196278034No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA920.1829171305869254No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA910.18092890090663272No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA880.17496421186575473No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA850.16899952282487674No Hit
ACTACGTACCGTAAAAAAAAAAAAAAAAAAAAA810.16104660410370605No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA790.15707014474312073No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA780.15508191506282806No Hit
CCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA760.15110545570224274No Hit
ACGCAGAGTGAATGGGCTACGTACCGAAAAAAA710.1411643073007794No Hit
GTGAATGGGCTACGTACAAAAAAAAAAAAAAAA680.1351996182599014No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA680.1351996182599014No Hit
CTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA660.13122315889931604No Hit
ACGCAGAGTGAATGGGCTACGTACCGTAAAAAA600.11929378081756005No Hit
TATCAACGCAGAGTGAATGGGCTACGTACCGTA550.1093526324160967No Hit
TCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA510.10139971369492604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT307.021453E-527.0019971
TATCATC307.0694325E-526.9750752
TGATACC1400.022.24681727
GAGTACG350.005458525419.2871381
TCTACGC350.005490163319.267913
ATCATCG350.005490163319.267913
TTGATAC1700.018.3209126
TATCAAC4100.017.1232171
ATCAACG4350.016.1230342
TCAACGC4450.015.1545363
TCTGCGT12050.014.72150927
GTATCAA2500.014.5810781
AACCTAC657.524782E-414.525046
TACCTAC657.524782E-414.525046
CAACGCA4800.014.0495184
ACCTACG2400.012.3635767
GTTGATA2550.011.682898525
CGTTGAT2550.011.682898524
AACGCAG5700.011.594555
CTCTGCG16650.010.73563726
Produced by FastQC (version 0.11.2)