FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_89.3520000005e19e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_89.3520000005e19e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11643
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATCGGTTGAACGTACTCTGCGTTGATACCA1130.9705402387700764No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA370.3177875118096711No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA360.3091986601391394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.24907669844541785No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA290.24907669844541785No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA270.23189899510435455No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA200.171777033410633No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA200.171777033410633No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT200.171777033410633No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA200.171777033410633No Hit
GTGAATGGGCCTATCGGAAAAAAAAAAAAAAAA190.16318818174010136No Hit
CCCTATCGGTTGAACGTACTCTGCGTTGATACC180.1545993300695697No Hit
GCCTATCGGTTGAACGTACTCTGCGTTGATACC180.1545993300695697No Hit
GCCTATCGGTAAAAAAAAAAAAAAAAAAAAAAA180.1545993300695697No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA170.14601047839903805No Hit
ACGCAGAGTGAATGGGCCTATCGGAAAAAAAAA150.12883277505797475No Hit
GCCCTATCGGTTGAACGTACTCTGCGTTGATAC150.12883277505797475No Hit
GAGTACGTTCAAAAAAAAAAAAAAAAAAAAAAA130.11165507171691144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT3350.020.95522327
AGCCTAT350.005213358419.2857157
TTCCTAT500.002071098716.1999997
CTCTGCG4650.015.38709526
ACTCTGC5050.013.9009925
CGTACTC5200.013.522
TATCAAC802.0816387E-413.51
ATCAACG802.0816387E-413.52
GTTGAAC5250.013.37142916
TTGAACG5250.013.37142917
GTACTCT5150.013.36893223
ACGTACT5200.013.24038421
TGAACGT5200.013.24038418
TACTCTG5100.013.23529324
TCCTATC1951.8189894E-1213.1538478
TCGGTTG5450.013.12844113
CGGTTGA5450.013.12844114
ATCGGTT5500.013.0090912
GGTTGAA5400.013.00000115
AACGTAC5300.012.99056620
Produced by FastQC (version 0.11.2)