FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_86.3520000005e14e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_86.3520000005e14e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6662
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCAACAACGTACT771.1558090663464426No Hit
ACAACGTACTCTGCGAAAAAAAAAAAAAAAAAA660.990693485439808No Hit
GTTCGGCAACAACGTACTCTGCGTTGATACCAC520.7805463824677273No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCAAAA420.6304413089162414No Hit
ACAACGTACTCTGCAAAAAAAAAAAAAAAAAAA420.6304413089162414No Hit
GTGAATGGGGTTGCCAAAAAAAAAAAAAAAAAA400.6004202942059442No Hit
GTATCAACGCAGAGTGAATGGGCAACAACGTAC350.5253677574302011No Hit
GTGAATGGGCAACAACGTACTCAAAAAAAAAAA320.48033623536475534No Hit
CAACAACGTACTCAAAAAAAAAAAAAAAAAAAA310.4653257280096067No Hit
ACAACGTACTCTGAAAAAAAAAAAAAAAAAAAA310.4653257280096067No Hit
GTGAATGGGCAACAACGTACTCTGAAAAAAAAA270.40528369858901225No Hit
ATCAACGCAGAGTGAATGGGCAACAACGTACTC260.39027319123386367No Hit
GAGTGAATGGGCAACAACGTACTCAAAAAAAAA240.3602521765235665No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCAAA240.3602521765235665No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA230.3452416691684179No Hit
CAACAACGTACTCTGAAAAAAAAAAAAAAAAAA230.3452416691684179No Hit
ACAACGTACTCTGCGTAAAAAAAAAAAAAAAAA230.3452416691684179No Hit
ACAACGTACTCAAAAAAAAAAAAAAAAAAAAAA220.3302311618132693No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGAA220.3302311618132693No Hit
GCAACAACGTACTCAAAAAAAAAAAAAAAAAAA220.3302311618132693No Hit
GTGAATGGGTGTTGCCAAAAAAAAAAAAAAAAA210.3152206544581207No Hit
GTGAATGGGTTGTTGCCAAAAAAAAAAAAAAAA210.3152206544581207No Hit
CTGTTCGGCAACAACGTACTCTGCGTTGATACC200.3002101471029721No Hit
CAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA190.2851996397478235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.2851996397478235No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGC180.2701891323926749No Hit
ACAACGTACTCTAAAAAAAAAAAAAAAAAAAAA180.2701891323926749No Hit
GGCAACAACGTACTCAAAAAAAAAAAAAAAAAA160.24016811768237767No Hit
GTGAATGGGCAACAACGTACTCTAAAAAAAAAA140.21014710297208045No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA140.21014710297208045No Hit
CAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA140.21014710297208045No Hit
CAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA130.19513659561693183No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTAAA130.19513659561693183No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA130.19513659561693183No Hit
ACGTACTCTAAAAAAAAAAAAAAAAAAAAAAAA130.19513659561693183No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCA130.19513659561693183No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCG130.19513659561693183No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGA120.18012608826178325No Hit
GTGAATGGGCAACAACGTACTCTGCGAAAAAAA120.18012608826178325No Hit
GGTATCAACGCAGAGTGAATGGGCAACAACGTA120.18012608826178325No Hit
AACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA110.16511558090663464No Hit
ACGTACTCTGCGTAAAAAAAAAAAAAAAAAAAA110.16511558090663464No Hit
GTGAATGGGGTTGTTGCCAAAAAAAAAAAAAAA110.16511558090663464No Hit
ACGTACTCTGCGAAAAAAAAAAAAAAAAAAAAA110.16511558090663464No Hit
ATGTTCGGCAACAACGTACTCTGCGTTGATACC110.16511558090663464No Hit
GTGTTCGGCAACAACGTACTCTGCGTTGATACC110.16511558090663464No Hit
AACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA110.16511558090663464No Hit
GTGAATGGGCAACAACGTACTCTGCGTAAAAAA110.16511558090663464No Hit
CAACGTACTCTGAAAAAAAAAAAAAAAAAAAAA100.15010507355148606No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA100.15010507355148606No Hit
GTGAATGGGTGTTCGGCAAAAAAAAAAAAAAAA100.15010507355148606No Hit
GTGAATGGGCAACAACGTACTCTGCAAAAAAAA100.15010507355148606No Hit
GAGTGAATGGGCAACAACGTACTCTAAAAAAAA100.15010507355148606No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT90.13509456619633745No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA90.13509456619633745No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCTG90.13509456619633745No Hit
GCAACAACGTACTCTGAAAAAAAAAAAAAAAAA90.13509456619633745No Hit
CAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA90.13509456619633745No Hit
AACGCAGAGTGAATGGGGTTGCCAAAAAAAAAA90.13509456619633745No Hit
ACGCAGAGTGAATGGGTTGTTGCCAAAAAAAAA90.13509456619633745No Hit
ACAACGTACTCTGCGTTAAAAAAAAAAAAAAAA80.12008405884118883No Hit
AACGCAGAGTGAATGGGGTTGTTGCCAAAAAAA80.12008405884118883No Hit
GAGTGAATGGGCAACAACGTACTCTGAAAAAAA80.12008405884118883No Hit
GTACGTTGTTGCCAAAAAAAAAAAAAAAAAAAA80.12008405884118883No Hit
ACGCAGAGTGAATGGGGTTGCCAAAAAAAAAAA80.12008405884118883No Hit
AACGCAGAGTGAATGGGTTGCCAAAAAAAAAAA80.12008405884118883No Hit
GAGTGAATGGGTGTTGCCAAAAAAAAAAAAAAA80.12008405884118883No Hit
GCAACAACGTACTCTGCGAAAAAAAAAAAAAAA80.12008405884118883No Hit
ACGTACTCTGAAAAAAAAAAAAAAAAAAAAAAA80.12008405884118883No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTAA80.12008405884118883No Hit
ACGCAGAGTGAATGGGTGTTGCCAAAAAAAAAA70.10507355148604022No Hit
TATCAACGCAGAGTGAATGGGTGTTGCCAAAAA70.10507355148604022No Hit
GAGTACGTTGTTGCCAAAAAAAAAAAAAAAAAA70.10507355148604022No Hit
CAACAACGTACTCTGCAAAAAAAAAAAAAAAAA70.10507355148604022No Hit
CAACAACGTACTCTAAAAAAAAAAAAAAAAAAA70.10507355148604022No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.10507355148604022No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC70.10507355148604022No Hit
ACGTACTCAAAAAAAAAAAAAAAAAAAAAAAAA70.10507355148604022No Hit
GTGAATGGGTTGCCAAAAAAAAAAAAAAAAAAA70.10507355148604022No Hit
GTGTTCGGCAAAAAAAAAAAAAAAAAAAAAAAA70.10507355148604022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA404.3380956E-420.251
TATCAAC350.00498248519.2857152
TCTGCGT1154.783942E-915.26086927
CGGCAAC1704.274625E-911.91176513
TGTTCGG1801.0029908E-811.259
TCGGCAA1851.5077603E-810.94594712
TTCGGCA1851.5077603E-810.94594711
GTTCGGC1851.5077603E-810.94594710
CTCTGCG1751.1776629E-610.02857126
GTGAATG1709.952906E-69.5294121
ACTCTGC1954.732212E-69.025
TGAATGG1554.6569554E-48.7096772
TACTCTG2252.9122652E-57.824
GGCAACA2652.8920767E-67.641509514
GCAACAA2703.7816972E-67.515
GTACTCT2458.473034E-57.16326523
AACAACG2901.0485244E-56.98275917
CAACAAC2901.0485244E-56.98275916
ACAACGT3052.141647E-56.63934418
CAACGTA3102.69348E-56.53225819
Produced by FastQC (version 0.11.2)