FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_85.3520000005e124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_85.3520000005e124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10984
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA1151.046977421704297No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA690.6281864530225784No Hit
CGGTACTAGAGACGTACTCTGCGTTGATACCAC680.6190823015294974No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.5644573925710124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.47341587764020393No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA420.3823743627093955No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA400.3641660597232338No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA350.31864530225782955No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA330.30043699927166784No Hit
GTGAATGGGTCGGTACAAAAAAAAAAAAAAAAA290.2640203932993445No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.2002913328477786No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA200.1820830298616169No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC170.15477057538237435No Hit
GTACTAGAGACGTACAAAAAAAAAAAAAAAAAA160.14566642388929352No Hit
GGTCGGTACAAAAAAAAAAAAAAAAAAAAAAAA150.13656227239621266No Hit
ACGCAGAGTGAATGGGTACTAGAGAAAAAAAAA140.12745812090313183No Hit
CTAGAGACGTACTCAAAAAAAAAAAAAAAAAAA140.12745812090313183No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA130.11835396941005098No Hit
GTGAATGGGTACTAGAGAAAAAAAAAAAAAAAA130.11835396941005098No Hit
GCTCGGTACTAGAGACGTACTCTGCGTTGATAC130.11835396941005098No Hit
AACGCAGAGTGAATGGGTCGGTACAAAAAAAAA120.10924981791697014No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10924981791697014No Hit
ACGCAGAGTGAATGGGTCGGTACAAAAAAAAAA120.10924981791697014No Hit
GTGAATGGGTCGGTACTAGAGAAAAAAAAAAAA120.10924981791697014No Hit
CTCTAGTACCAAAAAAAAAAAAAAAAAAAAAAA110.1001456664238893No Hit
TATCAACGCAGAGTGAATGGGTCGGTACAAAAA110.1001456664238893No Hit
CATCGGTACTAGAGACGTACTCTGCGTTGATAC110.1001456664238893No Hit
GTGAATGGGATCGGTACAAAAAAAAAAAAAAAA110.1001456664238893No Hit
CTTCGGTACTAGAGACGTACTCTGCGTTGATAC110.1001456664238893No Hit
TATCAACGCAGAGTGAATGGGGTCGGTACAAAA110.1001456664238893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA200.00828914926.9724771
AACTCAG200.00828914926.9724772
TCAGAGT200.00828914926.9724775
TACCGAA300.00213759922.4770647
TCTAGTA404.634258E-420.2293572
AGTACCG404.634258E-420.2293575
CTAGTAC404.634258E-420.2293573
CTCTAGT350.005226731819.2660541
GTATCAA509.276903E-518.8807351
GTACCGA450.001024361517.9816516
TAGTACC450.001024361517.9816514
TCTGCGT1251.020453E-915.17419227
TATCAAC750.002029646212.5871561
CTCTGCG1603.30092E-811.85483826
ATCAACG958.891344E-411.35683252
AATGGGG1257.678145E-49.7100924
ACTCTGC2007.249073E-79.48387125
TACTCTG2101.4129328E-69.03225824
GTACTCT2253.6105012E-68.43010823
CGTACTC2356.4989836E-68.0713822
Produced by FastQC (version 0.11.2)