FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_83.3520000005e0ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_83.3520000005e0ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24907
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC2450.9836592122696431No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.40149355602842574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.37338900710643597No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.140522744609949No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA320.12847793792909623No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC320.12847793792909623No Hit
GTGAATGGGGAGTCCTAAAAAAAAAAAAAAAAA280.1124181956879592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA504.7675712E-926.98594326
TGATACC504.7675712E-926.98594325
ATACCAC504.7675712E-926.98594327
CGCAAAG200.00847392126.9859437
GTTGATA551.2878445E-824.58203723
TTGATAC551.2878445E-824.58203724
TATCAAC1350.018.9901071
ATCAACG1350.017.9906272
TCAACGC1500.016.1915663
CGTTGAT1301.4006218E-1015.56881427
CAACGCA1650.015.5373624
AACGCAG1801.0004442E-1012.7433615
GTATCAA750.00215481212.5934411
TCTGCGT5150.011.26597627
ACGCAGA2305.0386006E-1010.5597176
ACAGTCC900.00841166710.4945347
CGCAGAG2357.348717E-1010.3350427
GCGTTGA2008.650204E-810.11972926
GCAGAGT2305.844413E-99.9730668
CAGAGTG2358.3200575E-99.7608739
Produced by FastQC (version 0.11.2)