FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_78.3520000005e04f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_78.3520000005e04f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6662
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA901.3509456619633744No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA370.5553887721404984No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA360.5403782647853498No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.5403782647853498No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA350.5253677574302011No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA270.40528369858901225No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA260.39027319123386367No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA260.39027319123386367No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT240.3602521765235665No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA220.3302311618132693No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA210.3152206544581207No Hit
ACGCAGAGTGAATGGGTTCGTCAAAAAAAAAAA200.3002101471029721No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA190.2851996397478235No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA150.22515761032722909No Hit
ACGCAGAGTGAATGGGTTCGTCACAAAAAAAAA140.21014710297208045No Hit
TATCAACGCAGAGTGAATGGGTTCGTCAAAAAA140.21014710297208045No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA130.19513659561693183No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA130.19513659561693183No Hit
AACGCAGAGTGAATGGGTTCGTCAAAAAAAAAA130.19513659561693183No Hit
GTTCGTCACACTACGTACTCAAAAAAAAAAAAA130.19513659561693183No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT120.18012608826178325No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCAAAAA120.18012608826178325No Hit
TTCGTCACACTACGTACTCTGCGTTGATACCAC120.18012608826178325No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA110.16511558090663464No Hit
CCGTTCGTCACACTACGTACTCTGCGTTGATAC110.16511558090663464No Hit
GTCACACTACGTACTCAAAAAAAAAAAAAAAAA110.16511558090663464No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA110.16511558090663464No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC110.16511558090663464No Hit
ACGCAGAGTGAATGGGTTCGTCACACTACGTAC100.15010507355148606No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA100.15010507355148606No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA100.15010507355148606No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA90.13509456619633745No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACACTA90.13509456619633745No Hit
ACACTACGTACTCAAAAAAAAAAAAAAAAAAAA90.13509456619633745No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACAAAA90.13509456619633745No Hit
GAGTGAATGGGTTCGTCAAAAAAAAAAAAAAAA90.13509456619633745No Hit
TCGTCACACTACGTACTCAAAAAAAAAAAAAAA80.12008405884118883No Hit
GTGAATGGGTTCGTCACACTACGTACAAAAAAA80.12008405884118883No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA80.12008405884118883No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA80.12008405884118883No Hit
ACGCAGAGTGAATGGGGTTCGTCAAAAAAAAAA80.12008405884118883No Hit
AACGCAGAGTGAATGGGTTCGTCACACTACGTA70.10507355148604022No Hit
CGTTCGTCACACTACGTACTCTGCGTTGATACC70.10507355148604022No Hit
GTATCAACGCAGAGTGAATGGGGTTCGTCACAC70.10507355148604022No Hit
ATCAACGCAGAGTGAATGGGTTCGTCAAAAAAA70.10507355148604022No Hit
GTGAATGGGGTTCGTCACACAAAAAAAAAAAAA70.10507355148604022No Hit
GTACGTAGTGTGACGAAAAAAAAAAAAAAAAAA70.10507355148604022No Hit
AATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA70.10507355148604022No Hit
GTCACACTACGTACAAAAAAAAAAAAAAAAAAA70.10507355148604022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT1500.017.127
CTCTGCG1900.013.526
ACTCTGC2001.8189894E-1212.82525
TACTCTG2051.8189894E-1212.51219524
CACACTA2200.012.27272715
TCACACT2200.012.27272714
GTACTCT2103.6379788E-1212.21428623
ACACTAC2250.012.016
CTACGTA2250.012.019
ACGTACT2153.6379788E-1211.93023321
CGTACTC2153.6379788E-1211.93023322
GTCACAC2301.8189894E-1211.73913113
TACGTAC2301.8189894E-1211.73913120
CACTACG2301.8189894E-1211.73913117
ACTACGT2301.8189894E-1211.73913118
CGTCACA2505.456968E-1210.812
TCGTCAC2505.456968E-1210.811
TTCGTCA2803.6379788E-1210.12510
GTTCGTC2803.6379788E-1210.1259
CGTTCGT1204.6565407E-410.1258
Produced by FastQC (version 0.11.2)