FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_76.3520000005dff0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_76.3520000005dff0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7803
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA1001.2815583749839805No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT610.7817506087402282No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.461361014994233No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA310.397283096245034No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA250.3203895937459951No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA230.2947584262463155No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA230.2947584262463155No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT210.2691272587466359No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA200.2563116749967961No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA200.2563116749967961No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA200.2563116749967961No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC180.2306805074971165No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA180.2306805074971165No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA170.2178649237472767No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA160.20504933999743688No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA160.20504933999743688No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA160.20504933999743688No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA150.1922337562475971No Hit
GTACGTAGAGGACGAAAAAAAAAAAAAAAAAAA130.16660258874791745No Hit
ACGCAGAGTGAATGGGATCTCGTCAAAAAAAAA120.15378700499807768No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCA120.15378700499807768No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA120.15378700499807768No Hit
AACGCAGAGTGAATGGGATCTCGTCCAAAAAAA110.14097142124823786No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCAAAA110.14097142124823786No Hit
GAGTGAATGGGATCTCGTCAAAAAAAAAAAAAA110.14097142124823786No Hit
GGTACGTAGAGGACGAAAAAAAAAAAAAAAAAA100.12815583749839804No Hit
ATCAACGCAGAGTGAATGGGATCTCGTCCAAAA100.12815583749839804No Hit
GAGTACGTAGAGGACGAAAAAAAAAAAAAAAAA90.11534025374855825No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA90.11534025374855825No Hit
GTAGAGGACGAAAAAAAAAAAAAAAAAAAAAAA80.10252466999871844No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCTC80.10252466999871844No Hit
CCCCCATCTCGTCCTCTACGTACTCTGCGTTGA80.10252466999871844No Hit
CGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10252466999871844No Hit
GATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAA80.10252466999871844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG300.002087941422.4679491
GCGTTGA805.174843E-716.95967727
GTATCAA905.341576E-411.9829061
TATCAAC905.341576E-411.9829061
TGCGTTG1307.88898E-510.43672526
TCAACGC1150.004146039.3779273
ATCAACG1150.004146039.3779272
CAACGCA1150.004146039.3779274
CTGCGTT1554.6684765E-48.75338325
TCTGCGT2202.214516E-68.63401827
GCAGAGT1700.00125358187.92986449
CTCTGCG2407.205659E-67.91451626
ACTCTGC2551.6284388E-57.44895625
CGCAGAG1850.0028904317.28690248
TACTCTG2652.723501E-57.16786424
GTACTCT2805.661858E-56.783870723
CGTACTC2857.155539E-56.664855522
ACGTACT2857.816966E-56.622132321
CTCTACG2909.8314784E-56.50795717
CCTCTAC2909.8314784E-56.50795716
Produced by FastQC (version 0.11.2)