FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_74.3520000005dfc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_74.3520000005dfc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5522
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACAGACAGTACGTACTCTGCGTTGATACCA2143.8754074610648317No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA821.4849692140528794No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT741.3400941687794277No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA490.8873596522998914No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA490.8873596522998914No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA440.796812749003984No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA420.7605939876856211No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA380.6881564650488953No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA360.6519377037305324No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA340.6157189424121695No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA300.5432814197754438No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA270.4889532777978993No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA270.4889532777978993No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA270.4889532777978993No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA260.4708438971387179No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.4708438971387179No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA250.45273451647953644No Hit
GTGAATGGGACACAAAAAAAAAAAAAAAAAAAA220.398406374501992No Hit
ATCAACGCAGAGTGAATGGGACACAGACAAAAA220.398406374501992No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA200.3621876131836291No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA200.3621876131836291No Hit
ACGCAGAGTGAATGGGACACAGAAAAAAAAAAA190.34407823252444764No Hit
TATCAACGCAGAGTGAATGGGACACAGAAAAAA190.34407823252444764No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC180.3259688518652662No Hit
GGACACAGACAGTACGTACTCTGCGTTGATACC180.3259688518652662No Hit
GAGTGAATGGGACACAGAAAAAAAAAAAAAAAA170.30785947120608476No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA160.2897500905469033No Hit
CGACACAGACAGTACGTACTCTGCGTTGATACC160.2897500905469033No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA150.2716407098877219No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGA140.2535313292285404No Hit
ACGCAGAGTGAATGGGGACACAGAAAAAAAAAA140.2535313292285404No Hit
GAATGGGACACAGAAAAAAAAAAAAAAAAAAAA130.23542194856935894No Hit
ATGGGACACAGACAAAAAAAAAAAAAAAAAAAA130.23542194856935894No Hit
ATCAACGCAGAGTGAATGGGACACAGAAAAAAA130.23542194856935894No Hit
GTGAATGGGGACACAGAAAAAAAAAAAAAAAAA130.23542194856935894No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA120.21731256791017745No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.21731256791017745No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA120.21731256791017745No Hit
CAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.21731256791017745No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.199203187250996No Hit
TATCAACGCAGAGTGAATGGGACACAAAAAAAA110.199203187250996No Hit
ACGCAGAGTGAATGGGACACAAAAAAAAAAAAA100.18109380659181454No Hit
AATGGGACACAGACAAAAAAAAAAAAAAAAAAA100.18109380659181454No Hit
GACACAGACAGTACGTACTCTTCGTTGATACCA100.18109380659181454No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA100.18109380659181454No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA100.18109380659181454No Hit
ACGCAGAGTGAATGGGGACACAGACAGAAAAAA100.18109380659181454No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA90.1629844259326331No Hit
CAACGCAGAGTGAATGGGACACAGACAAAAAAA90.1629844259326331No Hit
GAGTGAATGGGACACAAAAAAAAAAAAAAAAAA90.1629844259326331No Hit
GGACACAGACAAAAAAAAAAAAAAAAAAAAAAA90.1629844259326331No Hit
ATCAACGCAGAGTGAATGGGACACAGACAGAAA90.1629844259326331No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.1629844259326331No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.1629844259326331No Hit
GAGTGAATGGGACACAGACAGTACAAAAAAAAA90.1629844259326331No Hit
GAATGGGGACACAGACAGAAAAAAAAAAAAAAA90.1629844259326331No Hit
GAGTGAATGGGGACACAGACAAAAAAAAAAAAA80.14487504527345166No Hit
GAGTGAATGGGGACACAGAAAAAAAAAAAAAAA80.14487504527345166No Hit
AACGCAGAGTGAATGGGACACAGAAAAAAAAAA80.14487504527345166No Hit
ACAGTACGTACTCTGCGTTGATACCACTGCTTC80.14487504527345166No Hit
GTGAATGGGGGACACAGAAAAAAAAAAAAAAAA80.14487504527345166No Hit
ACGCAGAGTGAATGGGGACACAGACAAAAAAAA70.1267656646142702No Hit
CAACGCAGAGTGAATGGGACACAGACAGAAAAA70.1267656646142702No Hit
TATCAACGCAGAGTGAATGGGGACACAGAAAAA70.1267656646142702No Hit
GACACAGACAGTACGTAAAAAAAAAAAAAAAAA70.1267656646142702No Hit
GGTATCAACGCAGAGTGAATGGGACACAGACAG70.1267656646142702No Hit
GGTATCAACGCAGAGTGAATGGGACACAGACAA70.1267656646142702No Hit
AACGCAGAGTGAATGGGACACAAAAAAAAAAAA70.1267656646142702No Hit
TATCAACGCAGAGTGAATGGGGACACAGACAAA70.1267656646142702No Hit
GAATGGGACACAGACAGTACAAAAAAAAAAAAA60.10865628395508872No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA60.10865628395508872No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA60.10865628395508872No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA60.10865628395508872No Hit
TATCAACGCAGAGTGAATGGGGACACAGACAGA60.10865628395508872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA453.6266436E-521.00000224
GATACCA453.6266436E-521.00000227
TGATACC453.6266436E-521.00000226
TTGATAC453.6266436E-521.00000225
CGTTGAT551.6990823E-417.18181823
GCGTTGA603.3058206E-415.74999922
TGCGTTG800.00292299411.812521
CTGCGTT1000.001174655410.820
TCTGCGT1050.001767372810.28571419
CTCTGCG1200.00536291538.99999918
GACAGTA1658.109269E-48.1818187
AGACAGT1658.109269E-48.1818186
CAGTACG1500.00338985448.0999999
AGTACGT1500.00338985448.09999910
GTACGTA1500.00338985448.09999911
ACAGTAC1550.0045633017.83870948
CAGACAG1954.605017E-47.61538465
ACAGACA2551.2440044E-46.88235334
CACAGAC2551.2440044E-46.88235333
GACACAG2800.00300568675.78571371
Produced by FastQC (version 0.11.2)