FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_70.3520000005df43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_70.3520000005df43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18607
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA1901.0211210834632127No Hit
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC1670.897511689149245No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA1250.6717901864889557No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA1240.6664158649970441No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA980.5266835062073414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.5105605417316064No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC850.4568173268124899No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC820.440694362336755No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA810.4353200408448434No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA720.38695114741763853No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA670.3600795399580803No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA580.3117106465308755No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA560.30096200354705216No Hit
TATCTACGCAGAGTGAATGGGTAGTTCTGACAC550.2955876820551406No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA530.28483903907131725No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA500.26871607459558233No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA440.23647014564411242No Hit
GTATCAACGCAGAGTGAATGGGGTAGTTCTGAC430.23109582415220078No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA400.21497285967646584No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACA380.20422421669264254No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA380.20422421669264254No Hit
AGGTAGTTCTGACACGTACTCTGCGTTGATACC370.1988498952007309No Hit
CAACGCAGAGTGAATGGGTAGTTCTGACACAAA360.19347557370881927No Hit
GGTATCAACGCAGAGTGAATGGGTAGTTCTGAC360.19347557370881927No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA360.19347557370881927No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA350.18810125221690763No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGAAAA350.18810125221690763No Hit
ACGCAGAGTGAATGGGGTAGTTCAAAAAAAAAA340.18272693072499596No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACG320.1719782877411727No Hit
ACGCAGAGTGAATGGGTAGTTCAAAAAAAAAAA300.16122964475734938No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCAAAAA290.15585532326543775No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.15585532326543775No Hit
GAGTGAATGGGGTAGTTCAAAAAAAAAAAAAAA280.15048100177352608No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA270.14510668028161444No Hit
GAGTGAATGGGGTAGTTCTGACACAAAAAAAAA270.14510668028161444No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC260.1397323587897028No Hit
GTGAATGGGTAGTTCTGAAAAAAAAAAAAAAAA250.13435803729779117No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA250.13435803729779117No Hit
GTGAATGGGGTAGTTCTGACACGTACTCAAAAA240.1289837158058795No Hit
TGGTAGTTCTGACACGTACTCTGCGTTGATACC240.1289837158058795No Hit
GTGAATGGGGTAGTTCTGACACGTACTCTGCGT240.1289837158058795No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA220.11823507282205621No Hit
CAACTCAGAGTGAATGGGTAGTTCTGACACAAA220.11823507282205621No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGAAA210.11286075133014456No Hit
GTATCATCGCAGAGTGAATGGGGTAGTTCTGAC210.11286075133014456No Hit
GTGAATGGGGTAGTTCTGACACGTACAAAAAAA210.11286075133014456No Hit
GTGAATGGGTAGTTCTGACAAAAAAAAAAAAAA210.11286075133014456No Hit
GAATGGGTAGTTCTGACACAAAAAAAAAAAAAA200.10748642983823292No Hit
GACACGTACTCAAAAAAAAAAAAAAAAAAAAAA200.10748642983823292No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACAAAA200.10748642983823292No Hit
GGGTAGTTCTGACACGTACAAAAAAAAAAAAAA200.10748642983823292No Hit
AACGCAGAGTGAATGGGGTAGTTCTGACACGTA190.10211210834632127No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACA190.10211210834632127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA306.793426E-527.0000021
GTATCAT306.793426E-527.0000021
TATCATC306.793426E-527.0000022
ACTCAGA351.9361776E-423.1428593
TCAGAGT351.9361776E-423.1428595
CTCAGAG351.9361776E-423.1428594
TCGCAGA351.9361776E-423.1428597
ATCATCG351.9361776E-423.1428593
ATCGCAG351.9361776E-423.1428596
TCATCGC351.9361776E-423.1428594
CATCGCA351.9361776E-423.1428595
GTTGATA404.776652E-420.2525
TGATACC404.776652E-420.2527
AACTCAG404.776652E-420.252
TTGATAC404.776652E-420.2526
CGGGTAG753.3577453E-718.07
TATCAAC1253.6379788E-1217.2799991
CGTTGAT500.002137870516.224
TGCGTTG1800.015.027
TCTGCGT4500.013.227
Produced by FastQC (version 0.11.2)