FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_69.3520000005df29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_69.3520000005df29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7863
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.45784051888592137No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC240.3052270125906143No Hit
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA220.2797914282080631No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA210.2670736360167875No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA160.20348467506040951No Hit
TATCAACGCAGAGTGAATGGGCCAGTTCAAGGT150.19076688286913393No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA140.17804909067785832No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA140.17804909067785832No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA140.17804909067785832No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA130.16533129848658273No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA130.16533129848658273No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA120.15261350629530715No Hit
TCCCAGTTCAAGGTACGTACTCTGCGTTGATAC120.15261350629530715No Hit
GGTACGTACTCTGCGTTGATACAAAAAAAAAAA120.15261350629530715No Hit
GTACGTACTCTGCGTTGATACAAAAAAAAAAAA120.15261350629530715No Hit
GTGAATGGGCCAGTTCAAGGAAAAAAAAAAAAA120.15261350629530715No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA110.13989571410403154No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.13989571410403154No Hit
TATCAACGCAGAGTGAATGGGTACGTACTCTGC110.13989571410403154No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12717792191275595No Hit
CCAGTTCAAGGTACGTACTCTGCGTTGATACCA100.12717792191275595No Hit
GGCCAGTTCAAGGTAAAAAAAAAAAAAAAAAAA100.12717792191275595No Hit
GTATCAACGCAGAGTGAATGGGCCAGTTCAAGG100.12717792191275595No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11446012972148034No Hit
ACCCAGTTCAAGGTACGTACTCTGCGTTGATAC90.11446012972148034No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT90.11446012972148034No Hit
GCCCAGTTCAAGGTACGTACTCTGCGTTGATAC90.11446012972148034No Hit
GTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11446012972148034No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA90.11446012972148034No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT80.10174233753020476No Hit
ACGCAGAGTGAATGGGCCCAGTTCAAGGAAAAA80.10174233753020476No Hit
GTGAATGGGTACGTACTCTGCGTTGATACAAAA80.10174233753020476No Hit
CTCCAGTTCAAGGTACGTACTCTGCGTTGATAC80.10174233753020476No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA80.10174233753020476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCAGT200.00810865927.07
TCTGCGT2500.020.5227
AGCCAGT459.825821E-418.07
CTCTGCG3150.016.71428726
GCCAGTT1001.6980266E-816.28
ACTCTGC3500.015.04285625
TACTCTG3600.014.62524
AGGTACG3800.014.56578917
TCCAGTT656.5258186E-414.5384628
AAGGTAC3850.014.37662416
GTACTCT3750.014.03999923
CGTACTC3750.014.03999922
GGTACGT3950.014.01265818
ACGTACT3900.013.84615321
TTCAAGG4000.013.83750113
GTTCAAG4000.013.83750112
CAAGGTA4050.013.66666715
TCAAGGT4100.013.50000114
GTACGTA4150.013.33734919
CCAGTTC4050.013.3333339
Produced by FastQC (version 0.11.2)