FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_68.3520000005df0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_68.3520000005df0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10444
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGAGACTACGTACTCTGCGTTGATACCACT1701.6277288395250862No Hit
ACGTAGGAGACTACGTACTCTGCGTTGATACCA1571.5032554576790502No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA890.8521639218690157No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA720.689391037916507No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA620.5936422826503256No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA540.5170432784373803No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA490.4691689008042895No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA470.4500191497510532No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA470.4500191497510532No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.3925698965913443No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC410.3925698965913443No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA380.36384527001148986No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA300.2872462657985446No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA280.2680965147453083No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA270.25852163921869015No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA260.24894676369207203No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA250.23937188816545385No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA230.22022213711221755No Hit
GAATGGGACGTAGGAGAAAAAAAAAAAAAAAAA210.20107238605898123No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA210.20107238605898123No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA200.19149751053236308No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA200.19149751053236308No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA180.17234775947912676No Hit
GTGAATGGGACGTAGGAAAAAAAAAAAAAAAAA180.17234775947912676No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.17234775947912676No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAC170.1627728839525086No Hit
CCACGTAGGAGACTACGTACTCTGCGTTGATAC170.1627728839525086No Hit
GAGTGAATGGGACGTAGGAAAAAAAAAAAAAAA160.15319800842589046No Hit
AACGCAGAGTGAATGGGACGTAGGAGAAAAAAA160.15319800842589046No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAA150.1436231328992723No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.1436231328992723No Hit
ATCAACGCAGAGTGAATGGGACGTAGGAGACAA150.1436231328992723No Hit
GTGAATGGGGACGTAGGAAAAAAAAAAAAAAAA150.1436231328992723No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA150.1436231328992723No Hit
GACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAA140.13404825737265416No Hit
CAACGTAGGAGACTACGTACTCTGCGTTGATAC130.12447338184603601No Hit
GTATCATCGCAGAGTGAATGGGACGTAGGAGAC130.12447338184603601No Hit
CAACTCAGAGTGAATGGGACGTAGGAGACAAAA130.12447338184603601No Hit
ACGCAGAGTGAATGGGACGTAGGAAAAAAAAAA130.12447338184603601No Hit
CAACGCAGAGTGAATGGGACGTAGGAGACAAAA130.12447338184603601No Hit
CTACGTAGGAGACTACGTACTCTGCGTTGATAC130.12447338184603601No Hit
GTAGGAGACTACGTACTCTGCTTTGATACCACT130.12447338184603601No Hit
GTGAATGGGAACGTAGGAGAAAAAAAAAAAAAA130.12447338184603601No Hit
GTATCATCGCAGAGTGAATGGGACGTAGGAGAA120.11489850631941785No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA120.11489850631941785No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA120.11489850631941785No Hit
CAACGCAGAGTGAATGGGACGTAGGAGAAAAAA120.11489850631941785No Hit
CACGTAGGAGACTACGTACTCTGCGTTGATACC120.11489850631941785No Hit
ACACGTAGGAGACTACGTACTCTGCGTTGATAC110.10532363079279969No Hit
GCACGTAGGAGACAAAAAAAAAAAAAAAAAAAA110.10532363079279969No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA110.10532363079279969No Hit
CGTAGGAGACAAAAAAAAAAAAAAAAAAAAAAA110.10532363079279969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAG200.00827371626.9711553
GTATCAT306.5589404E-526.9711531
TATCATC306.5589404E-526.9711532
ATCATCG306.5589404E-526.9711533
TCGCAGA404.6157363E-420.2283657
ATCGCAG404.6157363E-420.2283656
TCATCGC404.6157363E-420.2283654
CATCGCA404.6157363E-420.2283655
GATACCA550.003780297714.78260727
TGATACC550.003780297714.78260726
TCTGCGT2057.18137E-910.57617627
GCGTTGA1150.00416897569.42659127
CTCTGCG2356.0397724E-89.22602626
ACGCAGA1701.3326373E-48.7259621
GCAGAGT1904.537645E-58.5172069
ACTCTGC2602.8691102E-78.33890725
TACTCTG2808.8948946E-77.743271424
CGCAGAG2101.4650331E-47.70604378
GTACTCT2951.961611E-67.34954623
CAGAGTG2050.00100575437.23616364
Produced by FastQC (version 0.11.2)